Isolasi dan Identifikasi Senyawa Metabolit Sekunder Ekstrak n-Heksan dari Umbi Lobak (Raphanus Sativus Lamk)

Ummi Zahra, Muharram Muharram, Asriani Ilyas Asriani Ilyas

Abstract


Isolation and identification of secondary metabolite compound in extract of n-Hexane in Umbi Lobak (Raphanus sativus Lamk) have been carried out. The purpose of this research is to know secondary metabolite compound which is contained in Umbi Lobak (Raphanus sativus Lamk) which obtained from Gowa. The compound was obtained by isolation process that consists of several steps including extraction, fractination, purification, and identification. Extraction was carried out using maceration with n- hexane. The identification has done by phytochemistry test, melting point, solubility, TLC, and IR spectroscopy. The result of research found out that the obtained compound was the steroid compound, with melt point 133-133,5 oC and had positive  reaction toward Lieberman Burchad reagents, where the Lieberman Burchad reagent resulting is green which perfectly solved in n-hexane, bit solved in methanol, and could not be solved in aceton. TLC analysis showed a stain in three scales different solvent and the result of elucidation structure in IR spectrophotometer showed that compound which get is β sitosterol.

Keywords


Raphanus Sativus; Secondary Metabolite; β Sitosterol

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References


Budiman, H., 2011,Tanaman Obat, http://www.iptek.net.id, 1 November 2011.

Firdaus, Z., 1999, Teknik Laboratorium Kimia Organik. Makassar: FMIPA Universitas Hasanuddin.

Harborne, J. B, 1987, Metode Fitokimia Penentuan Cara Modern Menganalisis Tumbuhan, Bandung: Institut Teknologi Bandung.

Istiqomah, N., 2011, Isolasi dan Identifikasi Beberapa Senyawa Flavonoid dalam Fase n-Butanol Dari Ekstrak Metanol Bunga Brokoli (Brassica oleracea L. var. botrytis L. Jakarta: Fakultas Farmasi Universitas Pancasila.

Kholifah, 2009, Dua Senyawa Terpenoid Alkohol Dari Rimpang Lengkuas Merah, Kalimantan Selatan: FMIPA Universitas Lambung Mangkurat.

Mahmiah, 2006, Isolasi dan Identifikasi Senyawa Flavonoid dari Kulit Batang Tumbuhan Saccopetalum horsfieldii Benn, Surabaya:Hang Tuah University.

Muharram, 1993, Beberapa Metabolit sekunder dari Cryptocarya Fusco-Pilosa Techner dan Cryptocarya ferrea BL (lauraceae), Bandung: Program Magister Kimia ITB.

Silverstein, 1991, Spectrometric Identification Of Organic compaunds, Edisi ke-5 Jhon Wiley dan Sons.

Sophi, D, 2012, Pengobatan Herbal Bibimbap Ampuh Gempur Batu Ginjal (Wawancara oleh Duto Sri Cahyono), Pengobatan Herbal Trubus, (12-05 2012).

Taofik, M, 2010, Isolasi dan Identifikasi Senyawa Aktif Ekstrak Air Daun Paitan (Thitonia Diversifolia) sebagai Bahan Insektisida Botani untuk Pengendalian Hama Tungau Eriophyidae, Malang:Fakultas Sains dan Teknologi UIN.

Pearson Wiliam Kwaku Ahianoi, 2004, Phytotoxin and Metabolism of Phytoalexins, Canada : Departement of chemistry, Universuti of Saskatchewan. h. 2




DOI: https://doi.org/10.24252/al-kimia.v1i1.1570

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