Biodegradasi Petroleum dan Hidrokarbon Eikosana oleh Isolat Bakteri Pseudomonas aeruginosa

Faiqah Umar

Abstract


Biodegradation of petroleum and hydrocarbon eicosane by Pseudomonas aeruginosa isolate. Hydrocarbon are important environmental contaminants in soil and water. These compounds have a potential risk to human health, as many of them are carsinogenic and toxic to marine organisms such as diatome, gasthrophode, mussel, and fish. The purpose of this research was to know the ability of Pseudomonas aeruginosa to degradate the hydrocarbon (petroleum Hundill and eicosane) substrate. Growing test used in two steps, the preculture and culture step. The biodegradation capacity was measured by quantitative and qualitative tests. The essay showed an increasing biodegradation capacitypercentage of bacteria cell mass on hydrocarbon substrate. The percentage on petroleum Hundill substrat as follows; log phase was 51,6%, descelerate phase was 73%, and linear phase was 81,4%. On eicosane substrate as follows; log phase was 62,7%, descelerate phase was 85,2%, and linear phase was 85,2%. The qualitative biodegradation capacity by chromatography result showed separate enchained of carbon n-alkana in each growth phase on petroleum Hundill substrate. Carbon chain termination as follows; C11, C12, C14, C15, C16, C18, C22 on log phase, C12, C17, C19, C20, C24 on descelerate phase, and C12 until C25 even better on linear phase.


Keywords


Biodegradation; Hydrocarbon; Quantitative; Qualitative

Full Text:

PDF

References


Atlas, R. M dan Bartha R., Degradation and Mineralization of Petroleum by Two Bacteria Isolated From Coastal Water, Biotechnology & Bioengeneering, vol XIV.

Atlas R. M., 1991, Microbial Hydrocarbon Degradation Bioremediation of Oil Spill, University of Louisville, Kentucky, USA, Review 52: 149-156.

Anhony, C, Wilbraham, 1992, Pengantar Kimia Organik Hayati, Bandung: Institut Teknologi Bandung.

Buttler, G. C., 1978, Principles Exotoxicology, John Wiley and Sons, New York.

Gunalan, 1993, Penerapan Bioremediasi Untuk Melenyapkan Polutan Organik dari Lingkungan, Palembang: Fakultas Pertanian, Universitas Sriwijaya.

Husain, D. R., Gonhy, Bezaec., 1997, Morphological Adaptation of Pseudomonas nautica Strain 617 to Growth on Eicosane and Mode of Eicosane Uptake.

Martani, Erni, 1992, Monograf Bioteknologi Lingkungan Pusat Antar Universitas (PAU), Yogyakarta: UGM.

Rheinheimer G., 1991, Aquatic Microbiolgy 4th Edition, John Wiley and Sons, New York.

Richard G. A., 1980, Marine Enviroment Pollution Hydrocarbon, New York: Elsevier Scientific Buplisher Company, Amsterdam, Oxford.

Suriawiria, U., 1986, Pengantar Mikrobilogi Umum, Bandung: Penerbit Angkasa Bandung.




DOI: https://doi.org/10.24252/al-kimia.v3i1.1662

Refbacks

  • There are currently no refbacks.


Al-Kimia has been indexed in :

See Google Scholar Profile for Al-Kimia by clicking here.

Mountain View     

More...

Supported By:

Creative Commons License All Publication by Al-Kimia are licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Visitor Number: StatCounter - Free Web Tracker and Counter View Al-Kimia Stats

________________________________________________
Al-Kimia, p-ISSN : 2302-2736, e-ISSN : 2549-9335
Faculty of Science and Technology, Alauddin State Islamic University of Makassar, Indonesia
Jl.H.M.Yasin Limpo No. 36 Samata, Gowa, Sulawesi Selatan
Phone: +6281-355-514-523, Email: al-kimia@uin-alauddin.ac.id