Preparation of Creatinine Sensor Material By Molecularly Imprinted Method Using MBAA and AMPSA as Precursors

Karmanto Karmanto

Abstract


Synthesis and preparation of creatinine sensor material by molecularly imprinted method using N,N'-Methylenebisacrylamide (MBAA) and 2-Acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) as precursors, on nylon membrane had been conducted. The precursor i.e. MBAA and AMPSA is used as cross-linker monomer, and functional monomer respectively, with the creatinine as template.  The performance of creatinine sensor material been studied by instrument response (resistance) pattern analysis to concentration of creatinine.  Result of FTIR spectra and SEM photograph, indicated the formation of molecularly imprinted polymer (MIP) on nylon membrane. Logarithmic pattern is appears as instrument response to concentration change of creatinine. Linear correlation of instrument response and creatinine concentration is observed at concentration ranges 1 to 5 ppm of creatinine. Comparative studies show that performance of nylon coated membrane is better than non-coated membrane. Linearity, limit detection, and limit quantification value of coated membrane is 0.9592; 1.2066 ppm; and 4.0221 ppm respectively. In other hand non-coated membrane give 0.8799; 2.1607 ppm and 7.2044 ppm respectively.

 

Keywords: Creatinine sensor, Molecularly imprinted polymer.

 

Abstrak

 

Sintesis dan preparasi material sensor kreatinin dengan metode molecularly imprinted menggunakan prekursor N,N'-Methylenebisacrylamida (MBAA) dan asam 2-Acrylamido-2-methyl-1-propanesulfonat (AMPSA) pada membran nylon telah berhasil dilakukan. Prekursor MBAA dan AMPSA masing-masing digunakan sebagai monomer cross-linker dan monomer fungsional dengan kreatinin sebagai template. Karakteristik kinerja material sensor kreatinin dikaji melalui analisis respon instrument terhadap perubahan konsentrasi kreatinin. Hasil analisis spektra FTIR dan fotografi SEM, menunjukan bahwa membran nilon telah terlapisi oleh material polimer MIP yang terbentuk dengan template molekul kreatinin.  Uji performa sensor menunjukkan bahwa respon sensor terhadap berbagai variasi konsentrasi sampel latih bersifat logaritmik dan memiliki rentang kerja (daerah linier) pada konsentrasi 0-5 ppm. Membran nylon MIP-kreatinin memiliki performa yang lebih baik daripada membran nylon tanpa modifikasi dengan nilai linieritas R2 sebesar 0,9592; limit deteksi 1,2066 ppm dan limit kuantifikasi 4,0221 ppm untuk membran nylon MIP kreatinin.

 

Kata kunci: Sensor kreatinin, Molecularly imprinted polymer.


Keywords


molecularly imprinted polymer, creatinine

References


Alexander C., Andersson H.S., Andersson L.I., Ansell R.J., Kirsch N., O’Mahony I.A.N., Whitcombe M.J., 2006, Molecular Imprinting Science and Technology: A Survey of The Literature for The Years Up to and Including 2003, J. Mol. Recognit, 19:106-180.

Baron, D.N., 1992, Patologi Klinik, diterjemahkan oleh Johannes Gunawan, Jakarta: Penerbit buku kedokteran EGC.

Choi, Y.S., Wang, K.H., Xu, M., dan Chung I.J., 2002, Synthesis of Exfoliated Polyacrylonitrile/Na-MMT Nanocomposites via Emulsion Polymerization, Chem. Mater, 14: 2936-2939.

Cormack, P.A.G. dan Elorza A.Z., 2004, Review Molecularly imprinted polymers: synthesis and characterization, J. Chromatogr, B 804 (2004) 173–182.

Doloksaribu, B., 2008, Pengaruh Proteksi Vitamin C terhadap Kadar Ureum, Kreatinin dan Gambaran Histopatologis Ginjal Mencit yang Dipapar Plumbum, (Skripsi), Medan: Universitas Sumatra Utara.

Farahmand, E., Ibrahim F., Hosseini S., Rothan H.A., Yusof R., Koole L.H., Djordjevic I., 2015, A Novel Approach for Application of Nylon Membranes in The Biosensing Domain, Applied Surface Science, 353: 1310–1319.

Fraden, Jacob, 2004, Handbook of Modern Sensors: Physics, Designs, and Applications 3rd Edition, New York: Springer-Verlag, Inc.

Harmita, 2004, Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitunganya, Majalah Ilmu Kefarmasian, Vol. I, No.3, 117 - 135.

Indonesian Renal Registry, 2015, 8th Report of Indonesian Renal Registry, www.indonesianrenalregistry.org., Diakses pada 1 Januari 2018.

Khadro, B., Sanglar C., Bonhomme A., Errachid A., Jaffrezic-Renault N., 2010, Molecularly Imprinted Polymers (MIP) Based Electrochemical Sensor for Detection of Urea and Creatinine, Procedia Engineering, 5: 371–374.

Khan, G.F. dan Wernet W, 1997, A Highly Snsitive Amperometric Creatinine Sensor, Anal Chim Acta, 351: 151-158.

Mattiasson, B. dan Lei Y, 2015, Molecularly Imprinted Polymers in Biotechnology, Switzerland: Springer International Publishing.

Pandey, R.P, Das A.K., Shahi V.K., 2015, 2 Acrylamido-2-methyl-1-propanesulfonic Acid Grafted Poly(vinylidene fluoride-co-hexafluoropropylene)-Based Acid-/ Oxidative-Resistant Cation Exchange for Membrane Electrolysis, ACS Appl. Mater. Interface, 7: 28524−28533.

Sergeyevaa, T.A., Gorbachb L.A., Piletska E.V., Piletskyc S.A., Brovkob O.O., Honcharovab L.A., Lutsykb O.D., Sergeevab L.M., Zinchenkoa O.A., El’skayaa A.V., 2013, Colorimetric Test-Systems for Creatinine Detection Based on Composite Molecularly Imprinted Polymer Membranes, Analytica Chimica Acta, 770: 161– 168.

Shon,Y-S., Wuelfing W.P., Murray R.W., 2001, Water-Soluble, Sulfonic Acid-Functionalized, Monolayer-Protected Nanoparticles and an IonicallyConductive Molten Salt Containing Them, Langmuir, 17: 1255-1261.

Wu, YH., dan Freeman B.D., 2009, Structure, Water Sorption, and Transport Properties of Crosslinked N-vinyl-2-pyrrolidone/ N,N-methylene bisacrylamide Films, Journal of Membrane Science, 344: 182–189.

Xue G., 1997, Fourier Transform Raman Spectroscopy and Its Application for The Analysis of Polymeric Materials, Prog. Polym. Sci., Vol. 22: 313-406.




DOI: https://doi.org/10.24252/al-kimia.v6i2.5592

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Al-Kimia, p-ISSN : 2302-2736, e-ISSN : 2549-9335
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