Total Phenolic and Flavonoid Content, and Antioxidant Capacity of Red Ginger (Zingiber officinale var. Rubrum) Ethanolic Extract and Fraction

Dwi Koko Pratoko


Determination of total phenolic, total flavonoid, antioxidant capacity from ethanolic extract and fractions of red ginger (Zingiber officinale var. Rubrum) has been done. Red ginger contain the highest oleoresins among other ginger varieties. Oleoresins are component that had the most responsibility for pharmacological effect of red ginger, one of them is as antioxidant. Phenolic and flavonoid are highly contribute to the antioxidant effect of red ginger. The aim of this research is to determine the antioxidant capacity of extract and fractions of red ginger, and to investigate the correlation between antioxidant capacity with both total phenolic and total flavonoid. Ethanolic extracts are fractinationed with n-hexane, chloroform, ethyl acetate, n-butanol, and methanol solvent. Antioxidant capacity, total phenolic, and total flavonoid of all the samples are measured. Highest antioxidant capacity, total phenolic, and total flavonoid obtained from ethyl acetate fraction with TEAC 2143,893 ± 0,890 µmol/g, GAE 229,878 ± 1,330 mg/g, and QE 46,564 ± 1,804 mg/g. One way ANOVA and post hoc analysis show significant result with p value <0,01. Pearson correlation shows high positive correlation.


Antioxidant capacity, red ginger, total flavonoid, total phenolic


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