The Effect of Extraction Methods on Antioxidant Activity of Acacia auriculiformis Stem Bark Ethanol Extract

Authors

  • Andri Anugrah Pratama Universitas Sembilanbelas November Kolaka
  • Muhammad Ashar Universitas Sembilanbelas November Kolaka
  • Adwhina Febry Yanty Universitas Sembilanbelas November Kolaka
  • Carla Wulandari Sabandar Universitas Sembilanbelas November Kolaka

DOI:

https://doi.org/10.24252/djps.v8i2.63007

Keywords:

Acacia auriculiformis, stem bark, extraction, antioxidant, DPPH

Abstract

Introduction: Acacia auriculiformis was recognized as a member of the Fabaceae family, and its stem bark exhibited pharmacological potential. The stem bark of Acacia auriculiformis has been traditionally used by native Australians to treat rheumatism. Aims: This study aimed to identify the chemical compounds and determine the effect of extraction methods on the antioxidant activity of the 70% ethanol extract of Acacia auriculiformis stem bark using the DPPH assay. Method: Antioxidant activity was expressed as the 50% scavenging concentration (SC50) and the Antioxidant Activity Index (AAI). The 70% ethanol extract of Acacia auriculiformis stem bark demonstrated antioxidant activity qualitatively through the dot-blot staining assay. Result: Phytochemical screening results showed that the Acacia auriculiformis stem bark obtained through maceration and reflux contained alkaloids, phenolics, flavonoids, and saponins. The antioxidant assay indicates antioxidant activity using the DPPH method in the stem bark extract of Acacia auriculiformis obtained through maceration and reflux, with SC₅₀ values of 2.08 μg/mL and 2.74 μg/mL, respectively, and AAI values of 19.20 and 14.61. Data analysis reveals no significant difference between the two extraction methods in terms of antioxidant activity, as determined by Tukey’s test (p ≥ 0.05). Conclusion: These findings indicate that both 70% ethanol extracts obtained through maceration and reflux exhibited antioxidant activity.

Downloads

Download data is not yet available.

References

Ahmadu, A. A., Lawal, B. A., Haruna, A., & Mustapha, L. (2019). Tetrahydroxy Flavone from Acacia auriculiformis A. Cunn ex benth. (Fabaceae) with novel kinase activity. Pharmacognosy Journal, 11(3), 559–563. https://doi.org/10.5530/pj.2019.11.89

Asworo, R. Y., & Widwiastuti, H. (2023). Pengaruh Ukuran Serbuk Simplisia dan Waktu Maserasi terhadap Aktivitas Antioksidan Ekstrak Kulit Sirsak. Indonesian Journal of Pharmaceutical Education, 3(2), 256–263. https://doi.org/10.37311/ijpe.v3i2.19906

Djuwarno, E. N., Abdulkadir, W. S., & Radjak, F. (2022). Uji Efektivitas Ekstrak Etanol Kulit Batang Kersen (Muntingia calabura L.) sebagai Antidiabetes pada Mencit Jantan (Mus musculus). Jambura Journal of Chemistry, 4(2), 47–55. https://doi.org/https://doi.org/10.37905/jambchem.v4i2.17510

Doloking, H., Mukhriani, M., Amalia, R., & Pratama, A. A. (2022). Antioxidant Activity Study of Zingiber Zerumbet Rhizome Extract and Fraction. Ad-Dawaa’ Journal of Pharmaceutical Sciences, 5(2), 120–129. https://doi.org/10.24252/djps.v5i2.37351

Girijashankar, V. (2011). Micropropagation of multipurpose medicinal tree Acacia auriculiformis. Journal of Medicinal Plants Research, 5(3), 462–466.

Johnson, E. C., Ukpe, R. A., Etim, E. I., Umoh, U. F., Eseyin, O. A., Udoh, A. E., … Anthony, P. C. (2021). In vitro antidiabetic, aphrodisiac and antimicrobial properties of para-propoxybenzoic acid isolated from Acacia auriculiformis A. Cunn Ex. Benth (Fabaceae) stem bark. Tropical Journal of Pharmaceutical Research, 20(3), 613–620. https://doi.org/10.4314/tjpr.v20i3.25

Jubaidah, S., Wijaya, H., Safira, A., & Ramadhan, M. M. (2024). Pengaruh Metode Ekstraksi terhadap Aktivitas Antioksidan Ekstrak Etanol Daun Singkil (Premna corymbosa Rottl. et Willd) dengan DPPH secara Spektrofotometri UV-Vis. Acta Holistica Pharmaciana, 6(1), 39–48. https://doi.org/10.62857/ahp.v6i1.160

Kaur, K., Arora, S., Hawthorne, M. E., Kaur, S., Kumar, S., & Mehta, R. G. (2002). A Correlative Study On Antimutagenic And Chemopreventive Activity Of Acacia Auricoliformis A. CUNN. and Acacia Nilotica (L.) Willd. ex Del. Drug and Chemical Toxicology, 25(1), 39–64. https://doi.org/10.1081/DCT-100108471

Kusuma, A. E., & Aprileili, D. A. (2022). Pengaruh Jumlah Pelarut Terhadap Rendemen Ekstrak Daun Katuk (Sauropus androgynus L. Merr). SITAWA : Jurnal Farmasi Sains Dan Obat Tradisional, 1(2), 125–135. https://doi.org/10.62018/sitawa.v1i2.22

Mutiar, S., Kasim, A., Emriadi, E., & Asben, A. (2018). Studi awal tanin dari kulit kayu Acacia auriculiformis A. Cunn. ex Benth. dari hutan tanaman industri untuk bahan penyamak kulit. Majalah Kulit, Karet, Dan Plastik, 34(2), 41–48. https://doi.org/10.20543/mkkp.v34i2.3967

Downloads

Published

2025-12-31

How to Cite

Pratama, A. A., Ashar, M., Yanty, A. F., & Sabandar, C. W. (2025). The Effect of Extraction Methods on Antioxidant Activity of Acacia auriculiformis Stem Bark Ethanol Extract. Ad-Dawaa’ Journal of Pharmaceutical Sciences, 8(2), 315–323. https://doi.org/10.24252/djps.v8i2.63007

Issue

Vol. 8 No.2 (2025)

Section

Artikel

License

Copyright (c) 2026 Andri Anugrah Pratama; Muhammad Ashar, Adwhina Febry Yanty, Carla Wulandari Sabandar

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Once an article was published in the journal, the author(s) are:

  • granted to the journal right licensed under Creative Commons License Attribution that allows others to share the work with an acknowledgement of the work's authorship.
  • permitted to publish their work online in third parties as it can lead to wider dissemination of the work.
  • continue to be the copyright owner and allow the journal to publish the article with the CC BY-SA license
  • receiving a DOI (Digital Object Identifier) of the work.