Isolation and Characterization of Amylolytic Bacteria From Peatlands Area in Central Kalimantan

Authors

  • Zahrotun Nafisah Universitas Palangka Raya
  • Syahrani Riana Dewi Universitas Palangka Raya
  • Naswa Ayu Andhita Universitas Palangka Raya
  • Enjel Elya Resha Universitas Palangka Raya
  • Efriyana Oksal Universitas Palangka Raya
  • Remi Ayu Pratika Universitas Palangka Raya
  • Dwi Hermayantiningsih Universitas Palangka Raya

Keywords:

Amylolytic bacteria, Amylase activity, pealand, ENS31, Central Kalimantan

Abstract

Peatlands in Central Kalimantan, Indonesia, are ecologically significant due to their acidic, organic-rich conditions, yet their microbial communities remain underexplored, particularly regarding their enzymatic potential. This study aimed to isolate and characterize amylolytic bacteria from peat soil collected at a depth of 143–150 cm in the LAHG CIMTROP area of Central Kalimantan. A total of 44 bacterial isolates were obtained using serial dilution and the gridding method. Screening for amylase activity identified isolate ENS31 as having the highest activity, shown by a clear halo on starch agar. ENS31 was further subjected to biochemical characterization and enzyme activity assays. The amylase enzyme exhibited optimal activity at pH 7 and 50°C, with a specific activity of 3.5 U/mg, and retained activity up to pH 12 (2 U/mg). These results indicate that ENS31 produces an enzyme stable in alkaline conditions despite originating from an acidic environment, demonstrating potential for industrial applications requiring pH and temperature-tolerant biocatalysts. This study contributes to understanding the functional diversity of peatland microbes and highlights tropical peat ecosystems as promising sources of novel enzymes.

 

Downloads

Download data is not yet available.

References

Erfianti, R., Kiranawati, T. M., & Rohajatien, U. (2023). Pengaruh Maltodekstrin Terhadap Sifat Fisik Dan Kimia Pewarna Bunga Tapak Dara (Catharanthus Roseus) Sebagai Biocolour Pangan. Jurnal Agroindustri, 13(1), 1–13. https://doi.org/10.31186/jagroindustri.13.1.1-13

Fauzi Akbar, Zulisma Anita, & Hamidah Harahap. (2013). Pengaruh Waktu Simpan Film Plastik Biodegradasi Dari Pati Kulit Singkong Terhadap Sifat Mekanikalnya. Jurnal Teknik Kimia USU, 2(2). https://doi.org/10.32734/jtk.v2i2.1431

Frima, F. K., Satiyarti, R. B., Anggraini, Y., Syafitri, E., & Rini, I. A. (2020). Characteristics of Raw-Starch Degrading Amylase Bacteria from Natar Hot Spring Lampung. Jurnal Kimia Sains Dan Aplikasi, 23(7), 238–243. https://doi.org/10.14710/jksa.23.7.238-243

Downloads

Published

2025-06-29 — Updated on 2025-07-30

Versions

How to Cite

Nafisah, Z., Riana Dewi, S., Ayu Andhita, N., Elya Resha, E., Oksal, E., Ayu Pratika, R., & Hermayantiningsih, D. (2025). Isolation and Characterization of Amylolytic Bacteria From Peatlands Area in Central Kalimantan. Al-Kimia, 13(1). Retrieved from https://journal.uin-alauddin.ac.id/index.php/al-kimia/article/view/55598 (Original work published June 29, 2025)

Issue

Vol. 13 No. 1 (2025): JUNE

Section

Article

License

Authors who publish with this journal agree to the following terms:

1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

3)Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).