Isolation and Characterization of Amylolytic Bacteria From Peatlands Area in Central Kalimantan

Authors

  • Zahrotun Nafisah Chemistry Departement, FMIPA, Universitas Palangka Raya https://orcid.org/0009-0003-3283-5176
  • Syahrani Riana Dewi Chemistry Departement, FMIPA, Universitas Palangka Raya
  • Naswa Ayu Andhita Chemistry Departement, FMIPA, Universitas Palangka Raya
  • Enjel Elya Resha Universitas Palangka Raya
  • Efriyana Oksal Universitas Palangka Raya
  • Remi Ayu Pratika Universitas Palangka Raya
  • Dwi Hermayantiningsih Universitas Palangka Raya

Keywords:

Amylolytic bacteria, Amylase activity, pealand, ENS31, Central Kalimantan

Abstract

Central Kalimantan has around 4.6 million hectares of peatland. Peat soil has organic content includes compounds such as humus, humic acid, fulvic acid, carbohydrates, lignin, proteins, and lipids, which influence the physical and chemical properties of the soil, including its water retention capacity, nutrient-holding ability, and support for microorganism growth. One of the common microbial activities found in peat soil is enzymatic activity, including amylolytic activity. Amylolytic bacteria are bacteria capable of producing amylase enzymes, which play a crucial role in the hydrolysis of starch in peatland. This study aims to isolate amylolytic bacteria from the peat soil of Central Kalimantan. There are 42 isolated pealand bacteria in this study. Furthermore, this study successfully isolated amylolytic bacterium ENS31 from peat soil, and showed that was able to produce amylolytic activity optimally at neutral pH and 50°C. The highest specific activity of amylase enzyme produced reached 3.5 U/mg. In addition, the amylase enzyme produced from ENS31 was able to maintain a specific activity up to 2 U/mg at pH 12, indicating its potential application in various industries, including waste treatment and biotechnology-based enzyme production.

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References

Erfianti, R., Kiranawati, T. M., & Rohajatien, U. (2023). Pengaruh Maltodekstrin Terhadap Sifat Fisik Dan Kimia Pewarna Bunga Tapak Dara (Catharanthus Roseus) Sebagai Biocolour Pangan. Jurnal Agroindustri, 13(1), 1–13. https://doi.org/10.31186/jagroindustri.13.1.1-13

Fauzi Akbar, Zulisma Anita, & Hamidah Harahap. (2013). Pengaruh Waktu Simpan Film Plastik Biodegradasi Dari Pati Kulit Singkong Terhadap Sifat Mekanikalnya. Jurnal Teknik Kimia USU, 2(2). https://doi.org/10.32734/jtk.v2i2.1431

Frima, F. K., Satiyarti, R. B., Anggraini, Y., Syafitri, E., & Rini, I. A. (2020). Characteristics of Raw-Starch Degrading Amylase Bacteria from Natar Hot Spring Lampung. Jurnal Kimia Sains Dan Aplikasi, 23(7), 238–243. https://doi.org/10.14710/jksa.23.7.238-243

Published

2025-06-29

How to Cite

Nafisah, Z., Dewi , S. R., Andhita, N. A., Elya Resha, E., Oksal, E., Ayu Pratika, R., & Hermayantiningsih, D. (2025). Isolation and Characterization of Amylolytic Bacteria From Peatlands Area in Central Kalimantan. Al-Kimia, 13(1). Retrieved from https://journal.uin-alauddin.ac.id/index.php/al-kimia/article/view/55598

Issue

Vol. 13 No. 1 (2025): JUNE

Section

Article

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