CHRYSIN MENINGKATKAN EFEK SITOTOKSIK TNF-RELATED APOPTOSIS-INDUCING LIGAND TERHADAP SEL HEK293
DOI:
https://doi.org/10.24252/jfuinam.v5i1.2366Abstract
TNF-related apoptosis-inducing ligand merupakan salah satu pilihan pengobatan kanker yang secara efektif dapat menginduksi apoptosis melalui aktivasi death receptor (DR4 dan DR5). Penggunaan TNF-related apoptosis-inducing ligand menyebabkan efek samping yang jauh lebih kecil dibandingkan dengan penggunaan kemoterapi. Namun saat ini beberapa jenis kanker menunjukkan resistensi terhadap TNF-related apoptosis-inducing ligand. Penggunaan TNF-related apoptosis-inducing ligand yang dikombinasikan dengan bahan alam menjadi salah satu alternatif untuk mengatasi resistensi yang timbul. Chrysin merupakan flavonoid yang memiliki efek antioksidan dan antikanker. Efek kombinasi TNF-related apoptosis-inducing ligand dan chrysin terhadap sel HEK293 ditentukan berdasarkan parameter viabilitas sel menggunakan metode WST-1. Chrysin tidak menyebabkan toksisitas yang besar terhadap sel HEK293 (<20%) namun setelah dikombinasikan dengan TNF-related apoptosis-inducing ligand menunjukkan peningkatan efek sitotoksik (41%) dan efek sinergi yang nyata (indeks sinergi = 0,69). Ini menunjukkan bahwa chrysin berpotensi untuk mengatasi resistensi terhadap TNF-related apoptosis-inducing ligand khususnya pada sel tumorigenik ginjal.
References
[1]Andrzejewski, T., Deeb, D., Gao, X., Danyluk, A., Arbab, A. S., Dulchavsky, S. A., & Gautam, S.C. Therapeutic efficacy of curcumin/TRAIL combination regimen for hormone-refractory prostate cancer. Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, 17(6), 257-267. 2008
[2]Ashkenazi, A., Holland, P., & Eckhardt, S. G. Ligand-based targeting of apoptosis in cancer: the potential of recombinant human apoptosis ligand 2/tumor necrosis factor–related apoptosis-inducing ligand (rhApo2L/TRAIL). Journal of Clinical Oncology, 26(21), 3621-3630. 2008
[3]Cho, H., Yun, C.-W., Park, W.-K., Kong, J.-Y., Kim, K. S., Park, Y., Kim, B.-K. Modulation of the activity of pro-inflammatory enzymes, COX-2 and iNOS, by chrysin derivatives. Pharmacological Research, 49(1), 37-43. 2004
[4]Fas, S. C., Baumann, S., Zhu, J. Y., Giaisi, M., Treiber, M. K., Mahlknecht, U., Li-Weber, M. Wogonin sensitizes resistant malignant cells to TNFα-and TRAIL-induced apoptosis. Blood, 108(12), 3700-3706. 2006
[5]Feng, Y., Wang, N., Zhu, M., Feng, Y., Li, H., & Tsao, S. Recent progress on anticancer candidates in patents of herbal medicinal products. Recent patents on food, nutrition & agriculture, 3(1), 30-48. 2011
[6]Fulda, S., & Debatin, K.-M. Resveratrol-mediated sensitisation to TRAIL-induced apoptosis depends on death receptor and mitochondrial signalling. European Journal of Cancer, 41(5), 786-798. 2005
[7]Gibson, S. B., Oyer, R., Spalding, A. C., Anderson, S. M., & Johnson, G. L. Increased expression of death receptors 4 and 5 synergizes the apoptosis response to combined treatment with etoposide and TRAIL. Molecular and cellular biology, 20(1), 205-212. 2000
[8]Guan, L. S., Li, G. C., Chen, C. C., Liu, L. Q., & Wang, Z. Y. Rb‐associated protein 46 (RbAp46) suppresses the tumorigenicity of adenovirus‐transformed human embryonic kidney 293 cells. International journal of cancer, 93(3), 333-338. 2001
[9]He, F., Wang, Q., Zheng, X.-L., Yan, J.-Q., Yang, L., Sun, H., Wang, X. Wogonin potentiates cisplatin-induced cancer cell apoptosis through accumulation of intracellular reactive oxygen species. Oncology reports, 28(2), 601-605. 2012
[10]Jemal, A., Bray, F., Center, M. M., Ferlay, J., Ward, E., & Forman, D. Global cancer statistics. CA: a cancer journal for clinicians, 61(2), 69-90. 2011
[11]Kavsan, V. M., Iershov, A. V., & Balynska, O. V. Immortalized cells and one oncogene in malignant transformation: old insights on new explanation. BMC cell biology, 12(1), 23. 2011
[12]Keane, M. M., Ettenberg, S. A., Nau, M. M., Russell, E. K., & Lipkowitz, S. Chemotherapy augments TRAIL-induced apoptosis in breast cell lines. Cancer research, 59(3), 734-741. 1999
[13]Khoo, B. Y., Chua, S. L., & Balaram, P. Apoptotic effects of chrysin in human cancer cell lines. International journal of molecular sciences, 11(5), 2188-2199. 2010
[14]Lee, E., Enomoto, R., Suzuki, C., Ohno, M., Ohashi, T., Miyauchi, A., Yokoi, T. Wogonin, a Plant Flavone, Potentiates Etoposide‐Induced Apoptosis in Cancer Cells. Annals of the New York Academy of Sciences, 1095(1), 521-526. 2007
[15]Lee, S.-J., Noh, H.-J., Sung, E.-G., Song, I.-H., Kim, J.-Y., Kwon, T. K., & Lee, T.-J. Berberine sensitizes TRAIL-induced apoptosis through proteasome-mediated downregulation of c-FLIP and Mcl-1 proteins. International journal of oncology, 38(2), 485. 2011
[16]Li, X., Wang, J.-N., Huang, J.-M., Xiong, X.-K., Chen, M.-F., Ong, C.-N., Yang, X.-F. Chrysin promotes tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induced apoptosis in human cancer cell lines. Toxicology in vitro, 25(3), 630-635. 2011
[17]Lin, Y.-C., Boone, M., Meuris, L., Lemmens, I., Van Roy, N., Soete, A., Drmanac, R. Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations. Nature communications, 5, 4767. 2014
[18]Parajuli, P., Joshee, N., Rimando, A. M., Mittal, S., & Yadav, A. K. In vitro antitumor mechanisms of various Scutellaria extracts and constituent flavonoids. Planta medica, 75(01), 41-48. 2009
[19]Sanderson, J. T., Hordijk, J., Denison, M. S., Springsteel, M. F., Nantz, M. H., & Van Den Berg, M. Induction and inhibition of aromatase (CYP19) activity by natural and synthetic flavonoid compounds in H295R human adrenocortical carcinoma cells. Toxicological Sciences, 82(1), 70-79. 2004
[20]Scambia, G., Ranelletti, F., Panici, P. B., Bonanno, G., De Vincenzo, R., Piantelli, M., & Mancuso, S. Synergistic antiproliferative activity of quercetin and cisplatin on ovarian cancer cell growth. Anti-cancer drugs, 1(1), 45-48. 1990
[21]Shen, C., Gu, M., Song, C., Miao, L., Hu, L., Liang, D., & Zheng, C. The tumorigenicity diversification in human embryonic kidney 293 cell line cultured in vitro. Biologicals, 36(4), 263-268. 2008
[22]Shi, R.-X., Ong, C.-N., & Shen, H.-M. Protein kinase c inhibition and x-linked inhibitor of apoptosis protein degradation contribute to the sensitization effect of luteolin on tumor necrosis factor–related apoptosis-inducing ligand–induced apoptosis in cancer cells. Cancer research, 65(17), 7815-7823. 2005
[23]Walczak, H., Miller, R. E., Ariail, K., Gliniak, B., Griffith, T. S., Kubin, M., Le, T. Tumoricidal activity of tumor necrosis factor–related apoptosis–inducing ligand in vivo. Nature medicine, 5(2), 157-163. 1999
[24]Wang, W., VanAlstyne, P. C., Irons, K. A., Chen, S., Stewart, J. W., & Birt, D. F. Individual and interactive effects of apigenin analogs on G2/M cell-cycle arrest in human colon carcinoma cell lines. Nutrition and cancer, 48(1), 106-114. 2004
[25]Woodman, O. L., & Chan, E. C. Vascular and anti‐oxidant actions of flavonols and flavones. Clinical and Experimental Pharmacology and Physiology, 31(11), 786-790. 2004
[26]Yamanaka, T., Shiraki, K., Sugimoto, K., Ito, T., Fujikawa, K., Ito, M., Suzuki, A. Chemotherapeutic Agents Augment TRAIL‐Induced Apoptosis in Human Hepatocellular Carcinoma Cell Lines. Hepatology, 32(3), 482-490. 2000
[27]Zhang, L., & Fang, B. Mechanisms of resistance to TRAIL-induced apoptosis in cancer. Cancer gene therapy, 12(3), 228-237. 2005
[2]Ashkenazi, A., Holland, P., & Eckhardt, S. G. Ligand-based targeting of apoptosis in cancer: the potential of recombinant human apoptosis ligand 2/tumor necrosis factor–related apoptosis-inducing ligand (rhApo2L/TRAIL). Journal of Clinical Oncology, 26(21), 3621-3630. 2008
[3]Cho, H., Yun, C.-W., Park, W.-K., Kong, J.-Y., Kim, K. S., Park, Y., Kim, B.-K. Modulation of the activity of pro-inflammatory enzymes, COX-2 and iNOS, by chrysin derivatives. Pharmacological Research, 49(1), 37-43. 2004
[4]Fas, S. C., Baumann, S., Zhu, J. Y., Giaisi, M., Treiber, M. K., Mahlknecht, U., Li-Weber, M. Wogonin sensitizes resistant malignant cells to TNFα-and TRAIL-induced apoptosis. Blood, 108(12), 3700-3706. 2006
[5]Feng, Y., Wang, N., Zhu, M., Feng, Y., Li, H., & Tsao, S. Recent progress on anticancer candidates in patents of herbal medicinal products. Recent patents on food, nutrition & agriculture, 3(1), 30-48. 2011
[6]Fulda, S., & Debatin, K.-M. Resveratrol-mediated sensitisation to TRAIL-induced apoptosis depends on death receptor and mitochondrial signalling. European Journal of Cancer, 41(5), 786-798. 2005
[7]Gibson, S. B., Oyer, R., Spalding, A. C., Anderson, S. M., & Johnson, G. L. Increased expression of death receptors 4 and 5 synergizes the apoptosis response to combined treatment with etoposide and TRAIL. Molecular and cellular biology, 20(1), 205-212. 2000
[8]Guan, L. S., Li, G. C., Chen, C. C., Liu, L. Q., & Wang, Z. Y. Rb‐associated protein 46 (RbAp46) suppresses the tumorigenicity of adenovirus‐transformed human embryonic kidney 293 cells. International journal of cancer, 93(3), 333-338. 2001
[9]He, F., Wang, Q., Zheng, X.-L., Yan, J.-Q., Yang, L., Sun, H., Wang, X. Wogonin potentiates cisplatin-induced cancer cell apoptosis through accumulation of intracellular reactive oxygen species. Oncology reports, 28(2), 601-605. 2012
[10]Jemal, A., Bray, F., Center, M. M., Ferlay, J., Ward, E., & Forman, D. Global cancer statistics. CA: a cancer journal for clinicians, 61(2), 69-90. 2011
[11]Kavsan, V. M., Iershov, A. V., & Balynska, O. V. Immortalized cells and one oncogene in malignant transformation: old insights on new explanation. BMC cell biology, 12(1), 23. 2011
[12]Keane, M. M., Ettenberg, S. A., Nau, M. M., Russell, E. K., & Lipkowitz, S. Chemotherapy augments TRAIL-induced apoptosis in breast cell lines. Cancer research, 59(3), 734-741. 1999
[13]Khoo, B. Y., Chua, S. L., & Balaram, P. Apoptotic effects of chrysin in human cancer cell lines. International journal of molecular sciences, 11(5), 2188-2199. 2010
[14]Lee, E., Enomoto, R., Suzuki, C., Ohno, M., Ohashi, T., Miyauchi, A., Yokoi, T. Wogonin, a Plant Flavone, Potentiates Etoposide‐Induced Apoptosis in Cancer Cells. Annals of the New York Academy of Sciences, 1095(1), 521-526. 2007
[15]Lee, S.-J., Noh, H.-J., Sung, E.-G., Song, I.-H., Kim, J.-Y., Kwon, T. K., & Lee, T.-J. Berberine sensitizes TRAIL-induced apoptosis through proteasome-mediated downregulation of c-FLIP and Mcl-1 proteins. International journal of oncology, 38(2), 485. 2011
[16]Li, X., Wang, J.-N., Huang, J.-M., Xiong, X.-K., Chen, M.-F., Ong, C.-N., Yang, X.-F. Chrysin promotes tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induced apoptosis in human cancer cell lines. Toxicology in vitro, 25(3), 630-635. 2011
[17]Lin, Y.-C., Boone, M., Meuris, L., Lemmens, I., Van Roy, N., Soete, A., Drmanac, R. Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations. Nature communications, 5, 4767. 2014
[18]Parajuli, P., Joshee, N., Rimando, A. M., Mittal, S., & Yadav, A. K. In vitro antitumor mechanisms of various Scutellaria extracts and constituent flavonoids. Planta medica, 75(01), 41-48. 2009
[19]Sanderson, J. T., Hordijk, J., Denison, M. S., Springsteel, M. F., Nantz, M. H., & Van Den Berg, M. Induction and inhibition of aromatase (CYP19) activity by natural and synthetic flavonoid compounds in H295R human adrenocortical carcinoma cells. Toxicological Sciences, 82(1), 70-79. 2004
[20]Scambia, G., Ranelletti, F., Panici, P. B., Bonanno, G., De Vincenzo, R., Piantelli, M., & Mancuso, S. Synergistic antiproliferative activity of quercetin and cisplatin on ovarian cancer cell growth. Anti-cancer drugs, 1(1), 45-48. 1990
[21]Shen, C., Gu, M., Song, C., Miao, L., Hu, L., Liang, D., & Zheng, C. The tumorigenicity diversification in human embryonic kidney 293 cell line cultured in vitro. Biologicals, 36(4), 263-268. 2008
[22]Shi, R.-X., Ong, C.-N., & Shen, H.-M. Protein kinase c inhibition and x-linked inhibitor of apoptosis protein degradation contribute to the sensitization effect of luteolin on tumor necrosis factor–related apoptosis-inducing ligand–induced apoptosis in cancer cells. Cancer research, 65(17), 7815-7823. 2005
[23]Walczak, H., Miller, R. E., Ariail, K., Gliniak, B., Griffith, T. S., Kubin, M., Le, T. Tumoricidal activity of tumor necrosis factor–related apoptosis–inducing ligand in vivo. Nature medicine, 5(2), 157-163. 1999
[24]Wang, W., VanAlstyne, P. C., Irons, K. A., Chen, S., Stewart, J. W., & Birt, D. F. Individual and interactive effects of apigenin analogs on G2/M cell-cycle arrest in human colon carcinoma cell lines. Nutrition and cancer, 48(1), 106-114. 2004
[25]Woodman, O. L., & Chan, E. C. Vascular and anti‐oxidant actions of flavonols and flavones. Clinical and Experimental Pharmacology and Physiology, 31(11), 786-790. 2004
[26]Yamanaka, T., Shiraki, K., Sugimoto, K., Ito, T., Fujikawa, K., Ito, M., Suzuki, A. Chemotherapeutic Agents Augment TRAIL‐Induced Apoptosis in Human Hepatocellular Carcinoma Cell Lines. Hepatology, 32(3), 482-490. 2000
[27]Zhang, L., & Fang, B. Mechanisms of resistance to TRAIL-induced apoptosis in cancer. Cancer gene therapy, 12(3), 228-237. 2005
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