Antiobesity Effect of NADES (Natural Deep Eutectic Solvent) Extract of Purslane Herb (Portulaca oleracea L.) on Male Rats (Rattus norvegicus)
Keywords:
Anti-obesity, green extraction, NADES, orlistat, Portuluca oleracea LAbstract
Introduction: Obesity is a metabolic disorder characterized by excessive fat accumulation, leading to various health complications. One potential approach to managing obesity is through natural products, such as medicinal plants. Aims: This study evaluates the anti-obesity activity of purslane (Portulaca oleracea L.) extract obtained using the green extraction method in male rats (Rattus norvegicus). Method: A total of 18 male rats were divided into six groups: normal control, negative control (Na-CMC + NADES), positive control (orlistat), and three treatment groups receiving NADES Purslane Herb Extract (NPHE) at doses of 50, 100, and 200 mg/kg BW. The treatment groups were induced with a high-fat diet for 30 days, followed by 14 days of therapy. Result: Statistical analysis using One-Way ANOVA and Bonferroni post-hoc test showed that NADES purslane herb extract exhibited significant anti-obesity effects, with the most effective dose being 200 mg/kg BW, which reduced body weight by 13.004% and BMI by 17.935% in male rats. Conclusion: These findings suggest that purslane extract has potential as a natural anti-obesity agent. Further research is needed to explore its bioactive compounds and mechanisms of action, supporting its potential development in pharmaceutical and nutraceutical applications.
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Ahmad, I., Prabowo, W. C., Nur, Y., Ardana, M., & Puji, B. (2020). Optimasi Metode Ekstraksi Berbantu Mikrowave dengan Pelarut Hijau (Asam Sitrat-Glukosa) Terhadap Kadar Polifenol Total dari Daun Kadamba (Mitragyna Speciosa Korth. Havil) Menggunakan Response Surface Methodology. Majalah Farmasi dan Farmakologi, 24(1), 11–16. https://doi.org/10.20956/mff.v24i1.9456
Azizah, R. N., Emelda, A., Asmaliani, I., Ahmad, I., & Fawwaz, M. (2022). Total Phenolic, Flavonoids, and Carotenoids Content and Anti-Obesity Activity of Purslane Herb (Portulaca oleracea L.) Ethanol Extract. Pharmacognosy Journal, 14(1), 8–13. https://doi.org/10.5530/pj.2022.14.2
Cheung, B. M. Y., Cheung, T. T., & Samaranayake, N. R. (2013). Safety of Antiobesity Drugs. Therapeutic Advances in Drug Safety, 4(4), 171–181. https://doi.org/10.1177/2042098613489721
Hikmawanti, A. P. E., Ramadhan, D., Ibrahim, L.-L., & Mun’im, A. (2021). Pelarut Eutectic Dalam Alami (NADES): Peningkat Kinerja Ekstraksi Fitokimia untuk Pengembangan Produk Farmasi dan Nutraceutical. Tanaman, 10(2091). https://doi.org/10.3390/ plant10102091
Hussein, M. A. (2010). Purslane Extract Effects on Obesity-Induced Diabetic Rats Fed A High-Fat Diet. Malaysian Journal of Nutrition, 16(3), 419–429.
Jung, J. H., Hwang, S. Bin, Park, H. J., Jin, G. R., & Lee, B. H. (2021). Antiobesity and Antidiabetic Effects of Portulaca oleracea Powder Intake in High-Fat Diet-Induced Obese C57BL/6 Mice. Evidence-Based Complementary and Alternative Medicine, 2021, 1–11. https://doi.org/10.1155/2021/5587848
Lee, J.I Li, J Fu, R. (2019). Evaluation of Anti Adipogenic Active Homoisofavonoids from Portulaca oleracea. Zeitschrift F¨ Ur Naturforschung C, 74(9–10), 265–273. https://doi.org/10.1515/znc-2018-0114
Liu, Y., Li, J., Fu, R., Zhang, L., Wang, D., & Wang, S. (2019). Enhanced Extraction of Natural Pigments from Curcuma Longa L. Using Natural Deep Eutectic Solvents. Ind. Crop. Prod, 140(111620). https://doi.org/10.1016/j.indcrop.2019.111620
Maimulyanti, A., Nurhidayati, I., Mellisani, B., Amelia Rachmawati Putri, F., Puspita, F., & Restu Prihadi, A. (2023). Development of Natural Deep Eutectic Solvent (NADES) Based on Choline Chloride As A Green Solvent To Extract Phenolic Compound from Coffee Husk Waste. Arabian Journal of Chemistry, 16(104634), 1–10. https://doi.org/10.1016/j.arabjc.2023.104634
Nemzer, B., Al-Taher, F., & Abshiru, N. (2020). Phytochemical Composition and Nutritional Value of Different Plant Parts in Two Cultivated and Wild Purslane (Portulaca Oleracea L.) Genotypes. Food Chemistry, 320(March). https://doi.org/10.1016/j.foodchem.2020.126621
Perumal, P. K., Huang, C. Y., Chen, C. W., Anisha, G. S., Singhania, R. R., Dong, C. Di, & Patel, A. K. (2023). Advances in Oligosaccharides Production from Brown Seaweeds: Extraction, Characterization, Antimetabolic Syndrome, and Other Potential Applications. Bioengineered, 14(1), 1–27. https://doi.org/10.1080/21655979.2023.2252659
Puspita, F., Nurhidayati, I., Mellisani, B., Putri, F. A. R., & Prihadi, A. R. (2023). Aplikasi Natural Deep Eutectic Solvent (NADES) sebagai Green Solvent pada Ekstraksi Kafein dan Katekin dari Limbah Kulit Kopi. Pharmaceutical and Biomedical Sciences Journal (PBSJ), 5(1), 35–42. https://doi.org/10.15408/pbsj.v5i1.32256
Riskesdas. (2018). Hasil Utama Riset Kesehatan Dasar Tahun 2018. Lembaga Penerbit Badan Penelitian dan Pengembangan Kesehatan.
Rosnah, R., Taslim, N. A., Aman, A. M., Idris, I., As’ad, S., Buchari, A., Bahar, B., Aminuddin, Wahyudin, E., & Nugraha, G. I. (2022). The Formulation and Evaluation of High-Fat Pellet on Lipid Profiles and Body Mass Index of Male Wistar Rats. Iraqi Journal of Pharmaceutical Sciences, 31(1), 285–292. https://doi.org/10.31351/vol31iss1pp285-292
Saputri, M., Lubis, S. H., Hasanah, F., Julianty, S. M., Fujiko, M., Ginting, E., Nasution, A. S., & Batubara, K. M. (2023). Effect of Sintrong Leaf Ethanol Extract (Crassocephalum crepidioides) On Weight Loss Of Male White Rats. Journal of Pharmaceutical and Sciences, 6(3), 1380-1385. https://doi.org/10.36490/journal-jps.com.v6i3.213
Septiyanti, & Seniwati. (2020). Obesitas dan Obesitas Sentral pada Masyarakat Usia Dewasa di Daerah Perkotaan Indonesia. Jurnal Ilmiah Kesehatan (JIKA), 2(3), 118–127. https://doi.org/https://doi.org/10.36590/jika.v2i3.74
Sutjiatmo, A. B., Haq, F. A., Jati, S. A., & Vikasari, S. N. (2021). The Effect of Ethanol Extract from Portulaca Oleracea on Inhibiting Total Cholesterol on Animalsubjects. Pharmacy Education. 21(2), 22-26. https://doi.org/10.46542/pe.2021.212.2226
Toar, J., Telew, A., & Lumenta, G. (2023). Perbedaan Tiga Kategori Aktifitas Fisik pada Status Obesitas dan Non Obesitas. HIGEIA (Journal of Public Health Research and Development), 7(3), 458–467. https://doi.org/10.15294/higeia.v7i3.61369
Ulfa, E. U., & Dianasari, D. (2024). Skrining Fitokimia dan Evaluasi Antibakteri Ekstrak NADES (Natural Deep Eutectic Solvents) Daun Iler (Coleus atropurpureus L. Benth) terhadap Bakteri Penyebab Folikulitis. Journal of Agropharmacy, 1(1), 1–5.
Vajdi, M., Hassanizadeh, S., Shojaei-Zarghani, S., Bagherniya, M., & Askari, G. (2023). The Effects of Purslane Consumption on Obesity Indices: A GRADE-Assessed Systematic Review and Meta-Analysis of Randomized Controlled Trials. Journal of Food Biochemistry, 2023(1), 1–11. https://doi.org/10.1155/2023/6655305
WHO. (2024). Obesity. Link : Https://Www.Who.Int/Health-Topics/Obesity., Diakses pada tanggal 18 Oktober 2024. https://www.who.int/health-topics/obesity
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