Antiinflammatory Activity of Methanolic Extract of Alpinia monopleura Rhizome In Vitro Using The HRBC Membrane Stabilization Method

Authors

  • Wahyuni Department of Pharmacy, Faculty of Pharmacy, Halu Oleo University, Kendari, Indonesia
  • Nur Af'idah Anas Magister of Pharmacy Program, Faculty of Pharmacy, Halu Oleo University, Kendari, Indonesia
  • Irnawati Department of Pharmacy, Faculty of Pharmacy, Halu Oleo University, Kendari, Indonesia
  • Muzuni Program Study of Biotechnology, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari, Indonesia
  • Adryan Fristiohady Department of Pharmacy, Faculty of Pharmacy, Halu Oleo University, Kendari, Indonesia
  • Suryani Department of Pharmacy, Faculty of Pharmacy, Halu Oleo University, Kendari, Indonesia
  • Loly Subhiaty Idrus Department of Pharmacy, Faculty of Pharmacy, Halu Oleo University, Kendari, Indonesia
  • Agung Wibawa Mahatva Yodha Polytechnic of Bina Husada, Kendari, Indonesia

DOI:

https://doi.org/10.24252/djps.v9i1.66284

Keywords:

Alpinia, anti-inflammatory, human red blood cell, zingiberaceae, in vitro

Abstract

Intoduction: Alpinia monopleura, a member of the Zingiberaceae family, is a plant species found in Southeast Sulawesi and has been traditionally used to alleviate muscle soreness. The rhizome contains bioactive compounds, including phenolics and flavonoids, which are believed to contribute to its anti-inflammatory activity. Aim: This study aimed to evaluate the anti-inflammatory activity of the methanolic extract of Alpinia monopleura rhizome. The anti-inflammatory activity was assessed in vitro using the Human Red Blood Cell (HRBC) membrane stabilization method, based on the extract’s ability to protect erythrocyte membranes against hypotonic solution-induced hemolysis. Result: The results demonstrated that the methanolic extract of Alpinia monopleura rhizome exhibited significant anti-inflammatory activity, as evidenced by its ability to stabilize red blood cell membranes. An increase in extract concentration corresponded to an increase in membrane stabilization. At the highest concentration tested, the methanolic extract of Alpinia monopleura rhizome showed 84.35% membrane stabilization, which was comparable to that of diclofenac sodium (88.16%). Conclusion: These findings suggest that the methanolic extract of Alpinia monopleura rhizome has promising potential for development as a natural anti-inflammatory agent.

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References

Aidoo, D. B., Konja, D., Henneh, I. T., & Ekor, M. (2021). Protective Effect of Bergapten against Human Erythrocyte Hemolysis and Protein Denaturation In Vitro. International Journal of Inflammation, 2021, 1–7. https://doi.org/10.1155/2021/1279359

Boonma, T., Saensouk, S., & Saensouk, P. (2023). Diversity and Traditional Utilization of the Zingiberaceae Plants in Nakhon Nayok Province, Central Thailand. Diversity, 15(8), 904. https://doi.org/10.3390/d15080904

Fristiohady, A., Malik, F., & Fariane, N. (2020). Uji Aktivitas Antiinfl amasi In Vitro Etlingera elatior (Jack) R. M. Smith dengan Metode Stabilisasi Membran Sel Darah. Jurnal Ilmu Kefarmasian Indonesia, 18(2), 150-157.

Fujiati, F., Haryati, H., Joharman, J., & Utami, S. W. (2022). In Vitro Metabolite

Profiling and Anti-Inflammatory Activities of Rhodomyrtus Tomentosa with Red Blood Cell Membrane Stabilization Methods. Reports of Biochemistry and Molecular Biology, 11(3), 502–510. https://doi.org/10.52547/rbmb.11.3.502

Hwang, Y.-J., Nam, S.-J., Chun, W., Kim, S. I., Park, S. C., Kang, C. D., & Lee, S. J. (2019). Anti-inflammatory Effects of Apocynin on Dextran Sulfate Sodium-Induced Mouse Colitis Model. PLOS ONE, 14(5), e0217642. https://doi.org/10.1371/journal.pone.0217642

Mir, R. H., & Masoodi, M. H. (2020). Anti-inflammatory Plant Polyphenolics and Cellular Action Mechanisms. Current Bioactive Compounds, 16(6), 809–817. https://doi.org/10.2174/1573407215666190419205317

Pandey, A. K. (2024). Quantitative Estimation of Secondary Metabolite, In-vitro Antioxidant, Anti-Sickling & Anti-Inflammatory Activity by HRBC Membrane Stabilization of Ethanolic Extract of Bacopa monnieri (L.) Pennell. Advances in Pharmacology and Pharmacy, 12(1), 19–33. https://doi.org/10.13189/app.2024.120103

Pangemanan, D. H. C. P., Mariati, N. W., & Rantetondok, A. L. (2024). Uji Aktivitas Anti-Inflamasi Ekstrak Sabut Kelapa (Cocos nucifera L.) dengan Metode Stabilisasi Membran Sel Darah Merah. E-GiGi, 13(1), 99–104. https://doi.org/10.35790/eg.v13i1.55338

Phull, A.-R., Ahmed, M., & Park, H.-J. (2022). Cordyceps militaris as a Bio Functional Food Source: Pharmacological Potential, Anti-Inflammatory Actions and Related Molecular Mechanisms. Microorganisms, 10(2), 405. https://doi.org/10.3390/microorganisms10020405

Rostom, B., Karaky, R., Kassab, I., & Sylla-Iyarreta Veitía, M. (2022). Coumarins derivatives and inflammation: Review of their effects on the inflammatory signaling pathways. European Journal of Pharmacology, 922, 174867. https://doi.org/10.1016/j.ejphar.2022.174867

Sulistyarto, S., Irawan, R., Kumaat, N. A., & Rimawati, N. (2022). Correlation of Delayed Onset Muscle Soreness and Inflammation Post-exercise Induced Muscle Damage. Open Access Macedonian Journal of Medical Sciences, 10(A), 1688–1694. https://doi.org/10.3889/oamjms.2022.10991

Wahyuni, W., Fristiohad, A., Sahidin, I., Mahatva Yo, A. W., Muh. Julia, L. O., Idrus, L. S., Y., M. I., Alaydrus, S., Sitti Musn, W. O., & Maulana, S. (2025). Identification of Potential Anticancer Bioactive Compounds from Fractions of Alpinia monopleura Rhizome Extract. Pakistan Journal of Biological Sciences, 28(4), 253–266. https://doi.org/10.3923/pjbs.2025.253.266

Yesmin, S., Paul, A., Naz, T., Rahman, A. B. M. A., Akhter, S. F., Wahed, M. I. I., Emran, T. Bin, & Siddiqui, S. A. (2020). Membrane Stabilization as a Mechanism of The Anti-Inflammatory Activity of Ethanolic Root Extract of Choi (Piper chaba). Clinical Phytoscience, 6(1), 59. https://doi.org/10.1186/s40816-020-00207-7

Yodha, A. W. M., Badia, E., Musdalipah, M., Setiawan, M. A., Daud, N. S., Fusvita, A., Fristiohady, A., & Sahidin, S. (2023). Essential Oils of Alpinia monopleura and Their Antibacterial and Antioxidant Activity. Molekul, 18(1), 80. https://doi.org/10.20884/1.jm.2023.18.1.6265

Yodha, A. W. M., Badia, E., Musdalipah, Reymon, Fauziah, Y., Fusvita, A., Arfan, Wahyuni, & Sahidin. (2024). Secondary Metabolite Compounds from Alpinia monopleura Extract and Evaluation of Anti-Inflammatory Activity based on In Vitro and In Silico Studies. HAYATI Journal of Biosciences, 31(6), 1154–1164. https://doi.org/10.4308/hjb.31.6.1154-1164

Youn, I., Han, A. R., Piao, D., Lee, H., Kwak, H., Lee, Y., Nam, J. W., & Seo, E. K. (2024). Phytochemical and pharmacological properties of the genus Alpinia from 2016 to 2023. Natural Product Reports, 1346–1367. https://doi.org/10.1039/d4np00004h

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Published

2025-06-30

How to Cite

Wahyuni, Nur Af’idah Anas, Irnawati, Muzuni, Adryan Fristiohady, Suryani, … Agung Wibawa Mahatva Yodha. (2025). Antiinflammatory Activity of Methanolic Extract of Alpinia monopleura Rhizome In Vitro Using The HRBC Membrane Stabilization Method. Ad-Dawaa’ Journal of Pharmaceutical Sciences, 9(1), 105–113. https://doi.org/10.24252/djps.v9i1.66284

Issue

Vol.9 No. 1 (2026)

Section

Artikel

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Copyright (c) 2026 Wahyuni, Nur Af'idah Anas, Irnawati, Muzuni, Adryan Fristiohady, Suryani, Loly Subhiaty Idrus, Agung Wibawa Mahatva Yodha

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