Uji Efektivitas Antibakteri Perasan Jus Buah Nanas (Ananas comosus) Terhadap Pertumbuhan Isolat Bakteri Plak Gigi di Puskesmas Kecamatan Tanah Abang Periode April 2017

Mitta Nurfitri Saridewi, Meiskha Bahar, Anisah Anisah

Abstract


Dental caries or commonly known as “Tooth Decay” is one of the most common infection on human. Dental caries begins by the formation of dental plaque and it is a multifactorial disorder. One of the efforts to prevent the formation of dental caries is by utilizing plants which contain antibacterial substance. Pineapple contains bromelain, saponin, flavonoid, and tannin enzymes which functions as antibacteria. This research aimed to discover the antibacterial effectiveness of pineapple juice toward the isolate growth of dental plaque bacteria by using three different pineapple juice concentrations (25%, 50%, and 75%). The method was experimental by using dental bacteria isolate from a patient of Dental and Oral Clinic in Puskesmas Kecamatan Tanah Abang. The result shows that the higher concentration shows the bigger inhibition zone diameter formed around the plate’s paper. Consequently in concentrations of 25%, 50%, and 75%, each has its own inhibition zone rate as much as 2,90 mm (weak); 3,89 mm (weak); and 5,89 mm (moderate).


Keywords


bromelain, Dental Plaque Bacteria Isolat, pineapple (Ananas comosus)

References


Allaker RP and Douglas CWI. 2009. Novel Anti-Microbial Therapies for Dental Plaque-Related Diseases. International Journal of Antimicrobial Agents. vol 33(1): 8-13. DOI: https://doi.org/10.1016/j.ijantimicag.2008.07.014.

Angraeni DP dan Rahmawati AD. 2012. Efektivitas daya antibakteri ekstrak kulit nanas (Ananas comosus (L) Merr) terhadap pertumbuhan Streptococcus mutans. [Skripsi]. Yogyakarta: Pendidikan Dokter Gigi Universitas Muhammadiyah Yogyakarta.

Au TK, Lam TL, Ng TB, Fong WP, Wan DCC. 2001. A comparison of HIV-1 integrase inhibition by aqueous and methanol extracts of Chinese medicinal herbs. Life Sciences. vol 68(14): 1687-1694. DOI: https://doi.org/10.1016/S0024-3205(01)00945-6.

Cahyono B. 2011. Buku Terlengkap Budidaya Nanas secara Komersial. Jakarta: Pustaka Mina.

Cushnie TPT and Lamb AJ. 2005. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents. vol 26(5): 343-356. DOI: https://doi.org/10.1016/j.ijantimicag.2005.09.002.

Dahlan MS. 2011. Statistik Untuk Kedokteran dan Kesehatan Edisi 5. Jakarta: Salemba Medika

Davis WW and Stout TR. 1971. Disc Plate Method of Microbiological Antibiotic Assay. Applied and Enviromental Microbiology. vol. 22 (4): 666-670.

Houwink, Dirks B, Winchel C. 2000. Ilmu Kedokteran Gigi Pencegahan. Yogyakarta: Gadjah Mada University Press.

Jo JY, de Mejia EG, Lila MA. 2005. Effects of Grape Cell Culture Extracts on Human Topoisomerase II Catalytic Activity and Characterization of Active Fractions. J. Agric. Food Chem, vol 53 (7): 2489–2498. DOI https://doi.org/10.1021/jf048524w.

Kidd EAM dan Bechal SJ. 2005. Dasar-dasar Karies: Penyakit dan Penanggulangannya. Jakarta: EGC.

Kongsuwan A, Suthiluk P, Theppakorn T, Srilaong V and Setha S. 2009. Bioactive compounds and antioxidant capacities of phulae and nanglae pineapple. As. J. Food Ag-Ind. vol 2, Special Issue: S44-S50.

Nurhidayah, Masriany, Masri M. 2013. Isolasi dan Pengukuran Aktivitas Enzim Bromelin dari Ekstrak Kasar Batang Nanas (Ananas comosus) Berdasarkan Variasi pH. Biogenesis. vol 1(2): 116-122. DOI: https://doi.org/10.24252/bio.v1i2.457.

Makalew MAJ, Nangoy E, Wowor PM. 2016. Uji Efek Antibakteri Air Perasan Daging Buah Nanas (Ananas comosus (L.) merr) Terhadap bakteri Klebsiella pneumonia. Jurnal e-Biomedik. vol 4(1): 1-6.

Parwata IMOA dan Dewi PFS. 2008. Isolasi dan Uji Aktivitas Antibakteri Minyak Atsiri Dari Rimpang Lengkuas (Alpinia galanga L.). Jurnal Kimia (Journal of Chemistry). vol 2(2): 100-104.

Pratiwi I. 2009. Uji Antibakteri Ekstrak Kasar Daun Acalypha indica Terhadap Bakteri Salmonella choleraesuis dan Salmonella typhimurium. [Skripsi]. Surakarta: Jurusan Biologi FMIPA Universitas Sebelas Maret.

Puskesmas Kecamatan Tanah Abang. 2016. Laporan Jumlah Pasien Pada Poli Gigi dan Mulut Tahun 2016. Jakarta Pusat.

Rakhmanda AP. 2008. Perbandingan Efek Antibakteri Jus Nanas (Ananas comosus L. merr) Pada Berbagai Konsentrasi Terhadap Streptococcus mutans. [KTI]. Semarang: Fakultas kedokteran, Universitas Diponegoro.

Riskesdas. 2007. Hasil Riset Kesehatan Dasar 2007. Jakarta: Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan RI. www.depkes.go.id. Diakses September 2017.

Riskesdas. 2013. Hasil Riset Kesehatan Dasar 2013. Jakarta: Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan RI. www.depkes.go.id. Diakses September 2017.

Wilson M. 2004. Lethal Photosensitisation of Oral Bacteria and Its Potential Application in the Photodynamic Therapy of Oral Infections. Photochem. Photobiol. Sci. vol 5(3): 412-418. DOI: https://doi.org/10.1039/B211266C.

World Health Organization. 2008. Oral Health Information Systems 2008. http://www.who.int. diakses Oktober 2017.




DOI: https://doi.org/10.24252/bio.v5i2.3532

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Biogenesis: Jurnal Ilmiah Biologi is Indexed by: 

                    

……………………………………………………………………………………………………………………………………………………

Biogenesis: Jurnal Ilmiah Biologi

pISSN 2302-1616
eISSN 2580-2909

Published by
Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar
Jl.H.M.Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia

Email: biogenesis@uin-alauddin.ac.id
WA: +6285242001984

……………………………………………………………………………………………………………………………………………………

Web Analytics 

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.