Efficacy of knockdown insecticide based on Permot (Passiflora foetida L.) leaf extract against mortality of German cockroach (Blattella germanica L.)

  • Rina Priastini Susilowati Department of Biology, Faculty of Medicine and Health, Universitas Kristen Krida Wacana
    (ID)
  • Flora Rumiati Department of Physiology, Faculty of Medicine and Health, Universitas Kristen Krida Wacana
    (ID)
Keywords: disease vector, knockdown time, neurotoxic efficacy, stinking passionflower, transfluthrin (TFT)

Abstract

Chemical insecticides have a detrimental effect on not only the target species, but also on non-target species and the environment. Transfluthrin is a pyrethroid class II insecticide that is considered to be safe for humans. However, repeated use over an extended period of time can result in cockroach resistance. For this reason, this study sought out alternative materials, specifically Permot leaf (Passiflora foetida L.). This study aims to determine the neurotoxic efficacy of insecticides derived from Permot leaf extract on German cockroach knockdown time and death. A total of 120 German cockroaches and was replicated three times. After 1 h of exposure to insecticides containing transfluthrin 3000 ppm and Permot leaf extract up to a dose of 4000 ppm, knockdown time and cockroach death were observed. The probit test was used to determine the cockroach knockdown time. The study's findings indicated that when cockroaches were exposed to insecticides derived from Permot leaves at a dose of 4000 ppm, the knockdown efficacy was KT50 for 8 min and KT90 for 30 min. The blocking test on German cockroach spiracles established that exposure via cockroach spiracles was more effective than exposure via other cockroach organs in killing.

References

Ajane GA, Patil AS. 2019. Evaluation of antioxidant potential of Passiflora foetida extract and quantitative evaluation of its phytochemical content-a possible natural antioxidant. The Pharmaceutical and Chemical Journal. vol 6(4): 14–24.

Arroyo-Maya IJ, Campos-Terán J, Hernández-Arana A, McClements DJ. 2016. Characterization of flavonoid-protein interactions using fluorescence spectroscopy: Binding of pelargonidin to dairy proteins. Food Chemistry. vol 213: 431–439. doi: https://doi.org/10.1016/j.foodchem.2016.06.105.

Asadujjaman M, Mishuk AU, Hossain MA, Karmakar UK. 2014. Medicinal potential of Passiflora foetida L. plant extracts: biological and pharmacological activities. Journal of Integrative Medicine. vol 12(2): 121–126. doi: https://doi.org/10.1016/S2095-4964(14)60017-0.

Asir PJ, Priyanga S, Hemmalakshmi S, Devaki K. 2014. In vitro free radical scavenging activity and secondary metabolites in Passiflora foetida L. Asian Journal of Pharmaceutical Research and Health Care. vol 6(2): 3–11. doi: https://doi.org/10.18311/ajprhc%2F2014%2F520.

Balabanidou V, Grigoraki L, Vontas J. 2018. Insect cuticle: a critical determinant of insecticide resistance. Current Opinion in Insect Science. vol 27: 68–74. doi: https://doi.org/10.1016/j.cois.2018.03.001.

Balabanidou V, Kefi M, Aivaliotis M, Koidou V, Girotti JR, Mijailovsky SJ, Juárez MP, Papadogiorgaki E, Chalepakis G, Kampouraki A, Nikolaou C, Ranson H, Vontas J. 2019. Mosquitoes cloak their legs to resist insecticides. Proceedings of the Royal Society B. vol 286(1907): 1–9. doi: https://doi.org/10.1098/rspb.2019.1091.

Bass C, Jones CM. 2016. Mosquitoes boost body armor to resist insecticide attack. Proceedings of the National Academy of Sciences. vol 113(33): 9145–9147. doi: https://doi.org/10.1073/pnas.1610992113.

Belete T. 2018. Defense mechanisms of plants to insect pests: from morphological to biochemical approach. Trends in Technical & Scientific Research. vol 2(2): 1–9. doi: https://doi.org/1019081/TTSR.2018.02.555584.

Brodowska KM. 2017. Natural flavonoids: classification, potential role, and application of flavonoid analogues. European Journal of Biological Research. vol 7(2): 108–123. doi: http://dx.doi.org/10.5281/zenodo.545778.

Chandelia S, Dubey NK. 2014. Mosquito repellent vaporizer poisoning–is the culprit transfluthrin or kerosene?. Indian Pediatrics. vol 51(4): 319–320. doi: https://doi.org/10.1007/s13312-014-0387-y.

Chinnasamy PS, Parimala S, Kandhasamy M. 2018. Phytochemical evaluation of seed and fruit pulp extracts of Passiflora foetida L. World Journal of Pharmaceutical Research. vol 7(7): 1924–1932. doi: https://doi.org/10.20959/wjpr20187-11770.

Dehghani R, Atharizadeh M, Moosavi SG, Azadi S, Rashidi M, Paksa A. 2014. Analysis of cockroach fauna and frequency in human residential habitats of North of Isfahan, Iran. International Archives of Health Sciences. vol 1(1): 25–29.

El-Sherbini GT, El-Sherbini ET. 2011. The role of cockroaches and flies in mechanical transmission of medical important parasites. Journal of Entomology and Nematology. vol 3(7): 98–104. doi: https://doi.org/10.5897/JEN.9000039.

Fardisi M, Gondhalekar AD, Ashbrook AR, Scharf ME. 2019. Rapid evolutionary responses to insecticide resistance management interventions by the German cockroach (Blattella germanica L.). Scientific Reports. vol 9(1): 1–10. doi: https://doi.org/10.1038/s41598-019-44296-y.

Fernando SD, Karunaratne MMSC. 2012. Ethnobotanicals for storage pest management: effect of powdered leaves of Olax zeylanica in suppressing infestations of rice weevil Sitophilus oryzae (Coleoptera: Curculionidae). Journal of Tropical Forestry and Environment. vol 2(1): 20–25. doi: https://doi.org/10.31357/jtfe.v2i1.38.

Finney DJ. 1971. Probit analisis. 3rd ed. London: Cambridge University Press. p 333.

Hikal WM, Baeshen RS, Said-Al Ahl HA. 2017. Botanical insecticide as simple extractives for pest control. Cogent Biology. vol 3(1): 1–16. doi: http://dx.doi.org/10.1080/23312025.2017.1404274.

Jang CW, Ju YR, Chang KS. 2017. Insecticide susceptibility of field‐collected Blattella germanica (Blattaria: Blattalidae) in Busan, Republic of Korea during 2014. Entomological Research. vol 47(4): 243–247. doi: https://doi.org/10.1111/1748-5967.12219.

Jensen K, Ko AE, Schal C, Silverman J. 2016. Insecticide resistance and nutrition interactively shape life-history parameters in German cockroaches. Scientific Reports. vol 6(1): 1–7. doi: https://doi.org/10.1038/srep28731.

Junayed M, Akter T, Ahmad S. 2020. Fresh leaf extract’s efficacy of twelve medicinal plants against Culex quinquefasciatus mosquito larvae. Journal of Science, Technology and Environment Informatics. vol 8(2): 606–617. doi: https://doi.org/10.18801/jstei.080220.62.

Kamal Z, Ullah F, Ahmad S, Ayaz M, Sadiq A, Imran M, Ahmad S, Rahman FU, Zeb A. 2017. Saponins and solvent extracts from Atriplex Laciniata L. exhibited high anthelmintic and insecticidal activities. Journal of Traditional Chinese Medicine. vol 37(5): 599–606. doi: https://doi.org/10.1016/S0254-6272(17)30312-6.

Mierziak J, Kostyn K, Kulma A. 2014. Flavonoids as important molecules of plant interactions with the environment. Molecules. vol 19(10): 16240–16265. doi: https://doi.org/10.3390/molecules191016240.

Nguyen TY, To DC, Tran MH, Lee JS, Lee JH, Kim JA, Woo MH, Min BS. 2015. Anti-inflammatory flavonoids isolated from Passiflora foetida. Natural Product Communications. vol 10(6): 929–931.

Nurcahya I, Hastutiek P, Damayanti R, Hamid IS, Wijaya A. 2020. In-vitro effectiveness of ethanol extract of Permot leaf (Passiflora foetida Linn.) towards mortality of Rhipicephalus sanguineus Larvae. Journal of Parasite Science. vol 4(2): 61–64. doi: http://dx.doi.org/10.20473/jops.v4i2.29155.

Olla G, Hasan T, Rupidara ADN. 2020. Effectiveness test of Rambusa (Passiflora foetida L.) fruit extract as a liquid anti-mosquito on the development vector of malaria mosquito (Anopheles sp.). Jambura Edu Biosfer Journal. vol 2(2): 44–50. doi: https://doi.org/10.34312/jebj.v2i2.2590.

Prasad R, Singh A, Mishra OP. 2015. An accidental ingestion of mosquito repellent liquid vapouriser. The Indian Journal of Pediatrics. vol 82(12): 1179–1180. doi: https://doi.org/10.1007/s12098-015-1788-1.

Rahayu R, Madona WR, Bestari W, Dahelmi D, Jannatan R. 2016. Resistance monitoring of some commercial insecticides to German cockroach (Blattella germanica (L.) in Indonesia. Journal of Entomology and Zoology Studies. vol 4(6): 709–712.

Pietri JE, Tiffany C, Liang D. 2018. Disruption of the microbiota affects physiological and evolutionary aspects of insecticide resistance in the German cockroach, an important urban pest. PloS One. vol 13(12): 1–17. doi: https://doi.org/10.1371/journal.pone.0207985.

Schapheer C, Sandoval G, Villagra CA. 2018. Pest cockroaches may overcome environmental restriction due to anthropization. Journal of Medical Entomology. vol 55(5): 1357–1364. doi: https://doi.org/10.1093/jme/tjy090.

Shahraki GH, Parhizkar S, Nejad AR. 2013. Cockroach infestation and factors affecting the estimation of cockroach population in urban communities. International Journal of Zoology. vol 2013: 1–7. doi: https://doi.org/10.1155/2013/649089.

Simirgiotis MJ, Schmeda-Hirschmann G, Bórquez J, Kennelly EJ. 2013. The Passiflora tripartita (Banana Passion) fruit: A source of bioactive flavonoid C-glycosides isolated by HSCCC and characterized by HPLC–DAD–ESI/MS/MS. Molecules. vol 18(2): 1672–1692. doi: https://doi.org/10.3390/molecules18021672.

Sisin NNT, Abdullah H, Suláin MD. 2017. Antiproliferative, antioxidative and compounds identification from methanolic extract of Passiflora foetida and its fractions. Journal of Analytical & Pharmaceutical Research. vol 6: 1–10. doi: http://dx.doi.org/10.15406/japlr.2017.06.00166.

Song Y, Wei XQ, Li MY, Duan XW, Sun YM, Yang RL, Su XD, Huang RM, Wang H. 2018. Nutritional composition and antioxidant properties of the fruits of a Chinese wild Passiflora foetida. Molecules. vol 23(2): 1–17. doi: https://dx.doi.org/10.3390%2Fmolecules23020459.

Sumita Y, Kawada H, Minakawa N. 2016. Mode of entry of a vaporized pyrethroid knockdown agent into the body of the housefly, Musca domestica (Diptera: Muscidae). Applied entomology and zoology. vol 51(4): 653–659. doi: https://doi.org/10.1007/s13355-016-0443-2.

Susilowati RP, Hartono B. 2017. Daya bunuh ekstrak daun Permot (Passiflora foetida) terhadap larva nyamuk Culex quinquefasciatus. Proceeding of Seminar Nasional Biologi XXIV. August 24-26, 2017. Manado: Universitas Sam Ratulangi. . ISBN 978-602-51854-0-3. pp 413-420.

Susilowati RP, Sari MP. 2018. Uji bioinsektisida ekstrak daun Permot (Passiflora foetida) terhadap kecoa Jerman (Blattella germanica). Proceeding of Seminar Nasional Biologi dan Pendidikan Biologi. January 20, 2018. Salatiga: Universitas Kristen Satya Wacana. ISBN 978-602-61913-1-1. pp 6–11.

Susilowati RP, Sari MP. 2021. Efek neurotoksik transflutrin, d-allethrin, dan ekstrak daun Permot (Passiflora foetida) terhadap kecoa Jerman (Blattella germanica). Proceeding of Seminar Nasional Riset Kedokteran (SENSORIK II). February 8, 2021. Jakarta: Universitas Veteran. pp 22–28.

Tandoro YO, Widyawati PS, Budianta TD, Sumargo GR. 2020. Phytochemical identification and antioxidant activity of passiflora foetida fruits and leaves extracts: A comparative study. International Journal of Pharmacy and Pharmaceutical Sciences. vol 12(6): 45–54.

Thawabteh A, Juma S, Bader M, Karaman D, Scrano L, Bufo SA, Karaman R. 2019. The biological activity of natural alkaloids against herbivores, cancerous cells and pathogens. Toxins. vol 11(11): 1–28. doi: https://doi.org/10.3390/toxins11110656.

Wu X, Appel AG. 2017. Insecticide resistance of several field-collected German cockroach (Dictyoptera: Blattellidae) strains. Journal of Economic Entomology. vol 110(3): 1203–1209. doi: https://doi.org/10.1093/jee/tox072.

Xiao J, Capanoglu E, Jassbi AR, Miron A. 2016. Advance on the flavonoid C-glycosides and health benefits. Critical Reviews in Food Science and Nutrition. vol 56(1): 29–45. doi: https://doi.org/10.1080/10408398.2015.1067595.

Xiong G, Tong X, Yan Z, Hu H, Duan X, Li C, Han M, Lu C, Dai F. 2018. Cuticular protein defective Bamboo mutant of Bombyx mori is sensitive to environmental stresses. Pesticide Biochemistry and Physiology. vol 148: 111–115. doi: https://doi.org/10.1016/j.pestbp.2018.04.005.

Zhu F, Lavine L, O’Neal S, Lavine M, Foss C, Walsh D. 2016. Insecticide resistance and management strategies in urban ecosystems. Insects. vol 7(1): 1–26. doi: https://doi.org/10.3390/insects7010002.

Zhukovskaya M, Novikova E, Saari P, Frolov RV. 2017. Behavioral responses to visual overstimulation in the cockroach Periplaneta americana L. Journal of Comparative Physiology A. vol 203(12): 1007-1015. doi: https://doi.org/10.1007/s00359-017-1210-8.

Zucolotto SM, Fagundes C, Reginatto FH, Ramos FA, Castellanos L, Duque C, Schenkel EP. 2012. Analysis of C‐glycosyl flavonoids from South American Passiflora species by HPLC‐DAD and HPLC‐MS. Phytochemical Analysis. vol 23(3): 232–239. doi: https://doi.org/10.1002/pca.1348.

Published
2021-12-30
Section
Research Articles
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