Lipid from microalgae isolated from Cengklik Reservoir, Boyolali, Central Java for prospective biodiesel sources

Authors

  • Siti Lusi Arum Sari Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret
  • Suratman Suratman Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret
  • Sunarto Sunarto Department of Environmental Science, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret
  • Tjahjadi Purwoko Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret
  • Dewi Larasati Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret
  • Stefanus Junliem Nugratama Herina Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret

DOI:

https://doi.org/10.24252/bio.v12i2.55910

Keywords:

Biodiesel, Chlorella sp., Lipid production, Microalgae, Oscillatoria sp., Scenedesmus sp.

Abstract

The availability and sustainability of the algae-based biodiesel industry depend on the adequate selection of microalgae strains. Based on this, it is important to explore local microalgae from Indonesia as a feedstock for biodiesel. This study aimed to isolate and screen freshwater microalgae with lipid-producing abilities. A total of three microalgal isolates have been successfully isolated from the Cengklik Reservoir (-7.506712, 110.723582). Based on the cell morphology, the three isolates were identified as Chlorella sp., Scenedesmus sp., and Oscillatoria sp. Nile red staining indicated that the three microalgal isolates contained neutral lipid droplets in their cells, as shown by the presence of a yellow or orange luminescence under a fluorescence microscope. The results of this study indicated that Scenedesmus sp. and Oscillatoria sp. had more potential as feedstocks for biofuels due to their higher lipid content and productivity. This research succeeded in obtaining microalgae isolates from Cengklik reservoir, Boyolali, Central Java which can be used as a source of lipids for biodiesel.

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Published

2025-08-02

Issue

Section

Research Articles