Screening of chitinase production medium by Fusarium solani isolated from endophytic karst using Two Level Factorial Design
DOI:
https://doi.org/10.24252/bio.v14i1.65554Keywords:
2-level factorial design, Chitinase, Fusarium solani, Response Surface Methodology, Screening mediumAbstract
Chitinase is an important enzyme in various biotechnology applications, especially in biological control, so it requires increased induction efficiency. This study aims to determine the growth media factors that most influence the production of chitinase enzymes by the endophytic fungus Fusarium solani using the 2-Level Fractional method. Eight media variables are estimated to have an effect, namely the content of chitin colloid, ammonium sulfate, potassium sodium tartrate, KH2PO4, Na2HPO4, MgSO4·7H2O, CaCl2, and pH. Chitinase activity was evaluated using the DNS technique after four days of liquid fermentation and analyzed using the design express DX6 4.1 application. The results of the analysis of variance (ANOVA) showed that the second-order polynomial regression model was very significant (p <0.0001), with an R² value of 0.9515, which indicates that the model explains 95.15% of the data variation. Based on the screening results, it was found that ammonium sulfate, Na2HPO4, and MgSO4 7H2 were the most effective in increasing chitinase production. Optimization of media conditions resulted in an increase in chitinase activity of up to 18 U/mL. This research has the potential to be used as a basis for developing industrial-scale bioprocesses for the formulation of chitinase-based biopesticides and biopharmaceutical products.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Musdalifah Musdalifah, Rachmawaty Rachmawaty, Alimuddin Alimuddin, Halifah Pagarra, Andi Asmawati Azis, Masriany Masriany
This work is licensed under a Creative Commons Attribution 4.0 International License.
COPYRIGHT AND LICENSE STATEMENT
COPYRIGHT
Biogenesis: Jurnal Ilmiah Biologi is published under the terms of the Creative Commons Attribution license. Authors hold the copyright and retain publishing rights without restriction to their work. Users may read, download, copy, distribute, and print the work in any medium, provided the original work is properly cited.
LICENSE TO PUBLISH
1. License
The use of the article will be governed by the Creative Commons Attribution license as currently displayed on http://creativecommons.org/licenses/by/4.0.
2. Author’s Warranties
The author warrants that the article is original, written by stated author/s, has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
Under the Creative Commons Attribution license, the users are free to download, reuse, reprint, modify, distribute and/or copy the content for any purpose, even commercially, as long as the original authors and source are cited. No permission is required from the authors or the publishers.
4. Co-Authorship
If the article was prepared jointly with other authors, the corresponding author warrants that he/she has been authorized by all co-authors, and agrees to inform his/her co-authors of the terms of this statement.
5. Miscellaneous
Biogenesis: Jurnal Ilmiah Biologi may conform the article to a style of punctuation, spelling, capitalization, and usage that it deems appropriate. The author acknowledges that the article may be published so that it will be publicly accessible and such access will be free of charge for the readers.
