SI PINTER Sebagai Alat Penghitung Koloni Bakteri Penunjang Laboratorium Mikrobiologi

  • Salsabila Yunita Kurniawan Airlangga University
    (ID)
  • Pancawati Ariami Politeknik Kesehatan Mataram
    (ID)
  • Rohmi Rohmi Politeknik Kesehatan Mataram
    (ID)
Keywords: bacterial colonies, colony count, SI PINTER

Abstract

Colony counter tools are used to count bacterial colonies. The high price constraint makes manual calculation the preferred alternative. In this study, Innovations were made to the SI PINTER tool by replacing the power source with a battery and using digital prayer beads connected to a marker to display the results on the screen. The purpose of this study was to develop the SI PINTER tool as a microbiology laboratory support to improve the accuracy of bacterial colony counts. Standardization of the SI PINTER tool is done by comparing the results of calculations using a conventional Colony Counter and without using equipment (manual) to get accurate results of the number of bacterial colonies. This research method is quasi-experimental and the data was analyzed by One Way ANOVA test. The results of the One Way ANOVA statistical test showed a p-value = 0.996 > 0.05, indicating that there was no significant difference between the results of the number of bacterial colonies using conventional Colony Counter, SI PINTER and manual calculations. The SI PINTER tool can be used according to its function as a microbial colony counter and has portable capabilities because it only relies on electrical energy from batteries

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References

Arhandi, P. P., Firdausi, A. T., & Pradana, D. (2019). Aplikasi Penghitung Koloni Bakteri Berbasis Android. JIP (Jurnal Informatika Polinema), 6, 23–32. https://doi.org/https://doi.org/10.33795/jip.v6i1.288

Aulanni’am. (2012). Instruksi Kerja Pemakaian Colony Counter. Universitas Brawijaya: Malang.

Cappuccino, J. G., & Sherman, N. (1998). Microbiology: A Laboratory Manual (ed. 5). Benjamin/Cummings Science Publishing. https://faculty.washington.edu/korshin/Class-486/MicrobiolTechniques.pdf

Dagelman, J. (1967). Colony Counter. Massachussetts: Cambridge. https://patentimages.storage.googleapis.com/cd/9e/3f/6d2b0e45c87fb6/US3344259.pdf

Damayanti, N. W. E., Abadi, M. F., & Bintari, N. W. D. (2020). Perbedaan Jumlah Bakteriuri Pada Wanita Lanjut Usia Berdasarkan Kultur Mikrobiologi Menggunakan Teknik Cawan Tuang Dan Cawan Sebar. Meditory, 8(1), 1–4. https://doi.org/10.33992/m.v8i1.969

Dwidjoseputro, D. (2005). Dasar-dasar Mikrobiologi (ed. 16). Djambatan: Jakarta. ISBN: 979-428-605-2.

Fardiaz, S. (1993). Analisis Mikrobiologi Pangan (Ed. 1 Cet.). PT Raja Grafindo Persada: Jakarta. ISBN: 979-413-769-3.

Harley, J. P., & Prescott, L. M. (2002). Laboratory Exercises To Accompany Microbiology (ed. 5). McGraw-Hill Publishing. ISBN: 0-07-282905-2.

Hastuti, U. S. (2018). Petunjuk Praktikum Mikrobiologi (ed. 2). UMM Press: Malang. ISBN: 978-979-796-330-9.

Hazan, R., Que, Y. A., Maura, D., & Rahme, L. G. (2012). A Method for High Throughput Determination of Viable Bacteria Cell Counts in 96-Well Plates. BMC Microbiology, 12(259), 1–7. https://doi.org/10.1186/1471-2180-12-259

Imas, M., & Nauri, A. (2018). Metodologi Penelitian Kesehatan (1st ed.). Kemenkes RI: Jakarta.

Irianto, K. (2014). Bakteriologi, Mikologi dan Virologi (ed. 1). Alfabeta: Bandung. ISBN: 978-602-289-051-5.

Jongenburger, I., Reij, M. W., Boer, E. P. J., Gorris, L. G. M., & Zwietering, M. H. (2010). Factors Influencing The Accuracy Of The Plating Method Used To Enumerate Low Numbers Of Viable Microorganisms In Food. International Journal of Food Microbiology, 143, 32–40. https://doi.org/10.1016/j.ijfoodmicro.2010.07.025

Kadri, A. N., Gelgel, K. T. P., & Suarjana, I. G. K. (2015). Perbedaan Cara Penyebaran Suspensi terhadap Jumlah Bakteri pada Media Eosin Methylene Blue Agar. Indonesia Medicus Veterinus, 4(3), 205–212. https://ojs.unud.ac.id/index.php/imv/article/view/17499

Murtius, W. S. (2018). Praktek Dasar Mikrobiologi. Universitas Andalas: Padang.

Notoatmodjo, S. (2012). Metodologi Penelitian Kesehatan (ed. 2). Rineka Cipta: Jakarta. ISBN: 978-979-518-984-8.

Pardede, S. O., Tambunan, T., Alatas, H., Trihono, P. P., & Hidayati, E. L. (2011). Konsensus Infeksi Saluran Kemih Pada Anak. In Ikatan Dokter Anak Indonesia. Ikatan Dokter Anak Indonesia (IDAI): Jakarta. ISBN: 9789798421648.

Rosmania, R., & Yanti, F. (2020). Perhitungan jumlah bakteri di Laboratorium Mikrobiologi menggunakan pengembangan metode Spektrofotometri. Jurnal Penelitian Sains, 22(2), 76. https://doi.org/10.56064/jps.v22i2.564

Rostavia, I., Ariswati., H. H. G., & Nugraha, P. C. (2016). Colony Counter Multipen. http://digilib.poltekkesdepkes-sby.ac.id/public/POLTEKKESSBY-Studi-723-DraftSeminar.pdf

Seniati, Marbiah, & Nurhayati. (2017). Kajian Uji Konfrontasi Terhadap Bakteri Pathogen dengan Menggunakan Metode Sebar, Metode Tuang dan Metode Gores. Jurnal; Galung Tropika, 6(1), 42–48. https://doi.org/10.31850/jgt.v6i1.209

Siragusa, M., Dall’olio, S., Fredericia, P. M., Jensen, M., & Groesser, T. (2018). Cell Colony Counter Called CoCoNut. PLoS ONE, 13(11), 1–18. https://doi.org/10.1371/journal.pone.0205823

Volk, W. ., & Wheeler, M. . (1993). Mikrobiologi Dasar (5th ed.). Erlangga: Jakarta.

Wicaksono, E. B., Hardianto, & Muliawan, A. (2019). Rancang Bangun Penghitung Jumlah Koloni Bakteri Berbasis Arduino Uno. Teknika, 13(2), 123–128. https://jurnal.polsri.ac.id/index.php/teknika/article/download/1787/965

Wijaya, R. C., Utari, E. L., & Yudianingsih. (2015). Perancangan Alat Penghitung Bakteri. Jurnal Teknologi Informasi, 10(29), 1–8. https://doi.org/10.35842/jtir.v10i29.138

WIPO. (1998). Colony Counter. Intellectual Property Organization: America https://doi.org/10.1126/science.183.4130.1229-c

Yunita, M., Hendrawan, Y., & Yulianingsih, R. (2015). Analisis Kuantitatif Mikrobiologi Pada Makanan Penerbangan (Aerofood ACS) Garuda Indonesia Berdasarkan TPC (Total Plate Count) Dengan Metode Pour Plate. Jurnal Keteknikan Pertanian Tropis Dan Biosistem, 3(3), 237–248. https://jkptb.ub.ac.id/index.php/jkptb/article/view/289/251

Yusmaniar, Wardiyah, & Nida, K. (2017). Bahan Ajar Farmasi: Mikrobiologi Dan Parasitologi (ed. 1). Kementerian Kesehatan Republik Indonesia: Jakarta.

Published
2023-06-30
How to Cite
Salsabila Yunita Kurniawan, Pancawati Ariami, & Rohmi Rohmi. (2023). SI PINTER Sebagai Alat Penghitung Koloni Bakteri Penunjang Laboratorium Mikrobiologi. Jurnal Biotek, 11(1), 87-97. https://doi.org/10.24252/jb.v11i1.35436
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Articles
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