ANALISIS NILAI ABSORBANSI UNTUK MENENTUKAN KADAR FLAVONOID DAUN JARAK MERAH (JATROPHA GOSSYPIFOLIA L.) MENGGUNAKAN SPEKTROFOTOMETER UV-VIS

Main Article Content

Ahriani Ahriani
Sri Zelviani
Hernawati Hernawati
Fitriyanti Fitriyanti

Abstract

Research on Absorbance Value Analysis on Determination of Flavonoid Levels in Red Jatropha Leaves (Jatropha Gossypifolia L.) using UV-Vis Spectrophotometer has been conducted. This study aimed to determine the absorbance of red jatropha (Jatropha Gossypifolia L.) leaves on young and old leaves and to determine the flavonoid content of red jatropha (Jatropha Gossypifolia L.) leaves on young and old leaves. The sample used in this study was 200 g of red jatropha leaf powder (Jatropha Gossypifolia L.) with the addition of 2000 ml of 70% ethanol as solvent in a ratio of 1:10. The extraction method used in this study is the MAE (Microwave Assisted Extraction) method to measure the absorbance value of the sample at a wavelength of 436 nm using a UV-Vis spectrophotometer. The results showed that the absorbance value of red jatropha (Jatropha Gossypifolia L.) leaves on young leaves was smaller than on old leaves. Where the absorbance value in young leaves is 0.355 while the absorbance value in old leaves is 0.616. The resulting absorbance value has complied with Lambert-Beer's law (0.2 ≤ A < 0.8). The flavonoid content of red jatropha (Jatropha Gossypifolia L.) leaves in young leaves was lower than in old leaves. Where the levels of flavonoids in young leaves is 2.71% while the levels of flavonoids in old leaves is 4.90%. This shows that the greater the absorbance value, the greater the flavonoid content produced.

Downloads

Download data is not yet available.

Article Details

How to Cite
Ahriani, A., Zelviani, S., Hernawati, H., & Fitriyanti, F. (2022). ANALISIS NILAI ABSORBANSI UNTUK MENENTUKAN KADAR FLAVONOID DAUN JARAK MERAH (JATROPHA GOSSYPIFOLIA L.) MENGGUNAKAN SPEKTROFOTOMETER UV-VIS. JFT: Jurnal Fisika Dan Terapannya, 8(2), 147-155. https://doi.org/10.24252/jft.v8i2.23379
Section
Artikel

References

Felicia Naomi. (2016). Pengaruh Ketuaan daun dan Metode Pengolahan terhadap Aktivitas Antioksidan Karakteristik Sensoris Teh Herbal Bubuk Daun Alpukat. Semarang: Universitas Wahid Hasyim.

Iqbal, Rustam Nurasisyah dan Kasman. 2015. Analisis Nilai Absorbansi Kadar Flavonoid Daun Sirih Merah (piper Crocatum) dan Daun Sirih Hijau (Piper Betle L). Gravitasi. Vol.15 No.1.

Neldawati, Ratnawulan dan Gusnedi. (2013). Analisis Nilai Absorbansi dalam Penentuan Kadar Flavonoid untuk Berbagai Jenis Daun Tanaman Obat. Jurnal Pillar of Physics.

Rachmani Eka Prasasti, Suwijoyo Pramono, Agung Endro Nogroho. (2018). Aktivitas Antioksidan Fraksi Flavonoid Bebas Andrografolid dari Herbal Sambiloto (Andrographis Paniculata). Pharmacy Medical Journal. Vol.1 No.2.

Salmia. (2016). Analisis Kadar Flavonoid Total Ekstrak Kulit Batang Kedondong Bangkok (Spondias dulcis) dengan Metode Spektrofotometri UV-Vis. (Skripsi), Makassar: UIN Alauddin Makassar.

Sembiring Timbangen, Dayana Indri, Rianna Martha. (2019). Alat Penguji Material. Bogor: Guepedia.

Warono Dwi dan Syamsudin. (2013). Unjuk Kerja Spektrofotometer Untuk Analisa Zat Aktif Ketoprofen. Jurnal Konversi 2.