Deteksi Gen Ketahanan Terhadap Powdery Mildew Pada Melon (Cucumis melo L. ‘Aramis’)

  • Budi Setiadi Daryono Jurusan Biologi, Fakultas Biologi Universitas Gadjah Mada Yogyakarta
  • Hani Christin Yambise Fakultas Biologi, Universitas Gadjah Mada


Melon (Cucumis melo L.) usually used as a fresh fruit, which contained high vitamin C and in Indonesia melon is one of the favorite fruit. Indonesia is a country that has a high biodiversity. One of the potential strategies in order to improve productivity and quality of crops competitiveness is through plant breeding. This study used melon Aramis from PT East West Seed Indonesia hybrid melon from crosses between 7319 (F) with 7347 (M). The research aims to determine the phenotype character of melon cultivar Aramis and to detect powdery mildew resistance gene. The research is conducted in Pusat Inovasi Agro Teknologi (PIAT UGM) Yogyakarta and at the Laboratory of Genetics and Breeding Faculty of Biology, Universitas Gadjah Mada. Resistance genes detection is conducted by Sequence Characterized Amplified Regions method (SCARs) using PCR and showed negative result of Aramis cultivar on detection of gene resistance to powdery mildew.


Abood JK, amd Lösel DM. 2003. Changes in carbohydrate composition of cucumber leaves during the development of powdery mildew infection. Plant Pathology. vol 52(2): 256–265.

Aristya GR, dan Daryono BS. 2013. Deteksi dan Skrining Pewarisan Sifat Ketahanan Penyakit Powdery Mildew pada Generasi Backcross Tanaman Melon (Cucumis melo L.) Var. Tacapa [Tesis]. Yogyakarta: Universitas Gadjah Mada.

Atak A. 2017. Determination of downy mildew and powdery mildew resistance of some grape cultivars. South African Journal of Enology and Viticulture. vol 38(1): 11–17.

Daryono BS, Aristya GR, Kasiamdari RS. 2011. Development of random amplified polymorphism DNA markers linked to powdery mildew resistance gene ini melon. Indonesian Journal of Biotechnology. vol 16(2): 76–82.

Daryono BS, dan Qurrohman MT. 2009. Pewarisan Sifat Ketahanan Tanaman Melon (Cucumis melo L.) terhadap Powdery Mildew (Podosphaera xanthii (Castag.) Braun et Shishkoff). Jurnal Perlindungan Tanaman Indonesia. vol 15(1): 1–6.

Fatmawati A, dan Daryono BS. 2016. Detection of Powdery Mildew (Podosphaera xanthii (Castagne) U. Braun & N. Shishkoff) Resistance Gene in Melon (Cucumis melo L.) Cultivar Tacapa Green Black. Bioengineering and Bioscience. vol 4(4): 51–55.

Fleige S, and Pfaffl MW. 2006. RNA integrity and the effect on the real-time qRT-PCR performance. Molecular Aspects of Medicine. vol 27(2–3): 126–139.

Fukino N, Kunihisa M, Matsumoto M. 2004. Characterization of Recombinant Inbred Lines Derived from Crosses in Melon (Cucumis melo L.), ‘PMAR No. 5’ × ‘Harukei No. 3.’ Breeding Science. vol 54(2): 141–145.

Gadoury DM, Seem RC, Pearson RC, Wilcox, WF, Dunst RM. 2001. Effects of Powdery Mildew on Vine Growth, Yield, and Quality of Concord Grapes. Plant Disease. vol 85(2): 137–140.

Huda IN, dan Daryono BS. 2013. Analisis Variasi Genetik Melon (Cucumis melo L.) Kultivar Gama Melon Basket Dengan Metode Random Amplified Polymorphic DNA. Biogenesis: Jurnal Ilmiah Biologi. vol 1(1): 41–50.

Lebeda A, and Mieslerová B. 2011. Taxonomy, distribution and biology of lettuce powdery mildew (Golovinomyces cichoracearum sensu stricto). Plant Pathology. vol 60(3): 400–415.

Li S, Paulsson M, Björn LO. 2002. Temperature-dependent formation and photorepair of DNA damage induced by UV-B radiation in suspension-cultured tobacco cells. Journal of Photochemistry and Photobiology B: Biology. vol 66(1): 67–72.

Listiawan DA. 2009. Deteksi Gen Pengkode Sifat Ketahanan Terhadap Powdery Mildew (Podosphaera xanthii [Castagne] U. Braun & N. Shishkoff) pada Melon (Cucumis melo L.) Dengan Penanda Molekuler Sequence Characterized Amplified Region (SCARs) [Skripsi]. Yogyakarta: Universitas Gadjah Mada.

McOrist AL, Jackson M, Bird AR. 2002. A comparison of five methods for extraction of bacterial DNA from human faecal samples. Journal of Microbiological Methods. vol 50(2): 131–139.

Pérez-García A, Romero D, FernÁndez-OrtuÑo D, López-Ruiz F, De Vicente A, TorÉ, JA. 2009. The powdery mildew fungus Podosphaera fusca (synonym Podosphaera xanthii), a constant threat to cucurbits: Pathogen profile. Molecular Plant Pathology. vol 10(2): 153–160.

Kementerian Pertanian. 2015. Statistik Produksi Hortikultura Tahun 2014. Jakarta: Kementerian Pertanian Direktorat Jenderal Hortikultura.

Sagova-Mareckova M, Cermak L, Novotna J, Plhackova K, Forstova J, Kopecky J. 2008. Innovative methods for soil DNA purification tested in soils with widely differing characteristics. Applied and Environmental Microbiology. vol 74(9): 2902–2907.

Stummer BE, Francis IL, Zanker T, Lattey K A, Scott ES. 2005. Effects of powdery mildew on the sensory properties and composition of Chardonnay juice and wine when grape sugar ripeness is standardised. Australian Journal of Grape and Wine Research. vol 11(1): 66–76.

Zhang QL, Liu JG, Chao H, Xue GQ, Ji LN. 2001. DNA-binding and photocleavage studies of cobalt(III) polypyridyl complexes: [Co(phen)2IP]3+ and [Co(phen)2PIP]3+. Journal of Inorganic Biochemistry. vol 83(1): 49–55.

Research Articles
Abstract viewed = 1506 times